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Research Article Open access CC BY 4.0

Differential Influence of Growth Regulators during Somatic Embryogenesis of Gynodioecious Papaya Varieties‘CO.7’ and ‘Red Lady

C. K. Rajesh, K. K. Kumar, C. Kavitha, G. Karthikeyan, K. Soorianathasundaram

Advances in Research · pp. 10–18 · Published 8 Apr 2020

10.9734/air/2020/v21i430196

Abstract

The study involved two auxins viz., 2,4-D (2,4-Diclorophenoxyacetic acid) and picloram at three different concentrations (1,2, 3 mg/L) in full strength MS media to study their comparative influence on induction of somatic embryogenesis from immature zygotic embryos of two gynodioecious varieties of papaya ‘CO.7’ and ‘Red Lady’. In papaya cultivar ‘CO.7’, 2,4-D at 2 mg/L gave the highest callus induction frequency of 90.93%, whereas comparatively higher concentration of 3 mg/L 2,4-D was found suitable for ‘Red Lady’ (87.26%). Although there was profuse callus formation, 2 mg/L 2,4-D recorded comparatively higher frequency of embryogenic calli in ‘Red Lady’ (51.67%) when compared to ‘CO.7’ (30.00%). Somatic embryo maturation was achieved upon transfer of embryogenic calli exhibiting globular stage embryos on to maturation medium (MS medium + ABA (Abscisic acid) and BAP (Benzyl amino purine) in different concentrations + glutamine 400 mg/L). In the maturation medium, the combination of 1.5 mg/L ABA and 0.4 mg/L BAP registered better conversion of the globular embryo to cotyledonary embryos than other levels. The frequency of somatic embryo germination was higher in ‘Red Lady’ (50.00%) as compared to ‘CO.7’ (31.67%) on half-strength MS medium devoid of growth regulators.

Somatic embryogenesis papaya picloram 2 4-Diclorophenoxyacetic acid CO.7 Red Lady immature zygotic embryos.

References (9)

  1. 1 Developmental pathways of somatic embryogenesis [DOI]
  2. 2 Zygotic embryogenesis versus somatic embryogenesis
  3. 3 QTLs and candidate genes for desiccation and abscisic acid content in maize kernels [DOI]
  4. 4 Production of triploid plants of papaya by endosperm culture [DOI]
  5. 5 High frequency somatic embryogenesis and plant regeneration from papaya hypocotyl callus [DOI]
  6. 6 Effects of abscisic acid on somatic embryo maturation of hybrid larch [DOI]
  7. 7 Induction of somatic embryogenesis in Papaya (Carica papaya)
  8. 8 Somatic embryogenesis from leaf explants of hermaphrodite Carica papaya : A new approach for clonal propagation
  9. 9 Comparison of the effects of kanamycin and geneticin on regeneration of papaya from root tissue [DOI]

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