Callus Induction from Carob (Ceratonia siliqua L.) Seedlings and Leaves of Mature Tree
Brahim El Bouzdoudi, R. Saïdi, Z. Nejjar El Ansari, M. Bouras, A. Badoc, A. Lamarti
Annual Research & Review in Biology · pp. 1–13 · Published 3 Nov 2017
10.9734/ARRB/2017/37037Abstract
Callus induction was successfully carried out from several explants of carob (Ceratonia siliqua L.). Callogenesis from the apex was tested on three different media containing Woody Plant Medium (WPM), Murashige and Skoog (MS) or Schenk and Hildebrandt (SH) macronutrients supplemented with two different hormonal solutions: benzylaminopurine (BAP) at 4.44 µM alone, or 2.22 µM of BAP plus 5 µM of 2-naphthalineacetic acid (NAA). Primary callus formation was obtained on a medium containing 88% WPM macronutrients. Callus formation from other parts of the plant was as follows: − Cotyledon embryos extracted from immature seeds (85% success rate on WPM medium, containing 4.44 µM BAP and 5 µM NAA); − Cotyledon leaves taken from 7-day-old seedlings, obtained from in vitro germination of seeds (62% success rate on WPM medium, containing 4.44 µM BAP and 5 µM NAA); − Hypocotyls taken from 7-day-old seedlings (55% success rate on WPM containing 2.22 µM BAP and 5 µM NAA); − Differentiated leaves taken from mature tree (84% success rate on WPM medium, containing 4.44 µM of BA and 2.26 µM of NAA). In general, production of primary calli and their growth after transplantation was better on WPM medium supplemented with 2.5 µM NAA and 2.22 µM BAP.
References (24)
- 1 A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures [DOI]
- 2 Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures [DOI]
- 3 Carob tree, Ceratonia siliqua L.
- 4 Fruit and seed diversity of domesticated carob (Ceratonia siliqua L.) in Morocco [DOI]
- 5 Somatic embryogenesis from stem and leaf explants of Quercus robur L. [DOI]
- 6 Analysis of shoot-forming capacity in vitro in two lines of tomato (Lycopersicon esculentum Mill.) and their hybrids 1 [DOI]
- 7 Microsporogenesis and anther culture in carob tree (Ceratonia siliqua L.) [DOI]
- 8 Effect of growth regulators and explant origin on in vitro propagation of Ceratonia siliqua L. via cuttings
- 9 Clonal propagation of Carob (Ceratonia siliqua L., Fabaceae) [DOI]
- 10 A micropropagation system for carob (Ceratonia siliqua L.) [DOI]
- 11 High incidence regeneration system in Ceratonia siliqua L. articulated with SEM and biochemical analysis during developmental stages [DOI]
- 12 In vitro propagation of Moroccan carob (Ceratonia siliqua L.)
- 13 Factors affecting shoot initiation from tuber discs of potato (Solanum tuberosum) [DOI]
- 14 PRODUCTION OF ANOMALOUS STRUCTURES IN QUERCUS RUBRA L. CALLUS CULTURES [DOI]
- 15 Effect of Phloroglucinol on Shoot Growth and Initiation of Roots in Carob Tree Cultures Grown In Vitro [DOI]
- 16 Callus induction and somatic embryogenesis in carob (Ceratonia siliqua L.) from ovule culture [DOI]
- 17 Somatic embryo production from immature seeds of carob (Ceratonia siliquaL.): histological evidence [DOI]
- 18 Tissue Culture of Douglas-Fir and Western North American Conifers [DOI]
- 19 Establishment of Proliferating Callus from Roots, Cotyledons and Hypocotyls of Carob (Ceratonia siliqua L) Seedlings [DOI]
- 20 Callogenesis of Cork Oak (Quercus suber L.) through In Vitro Culture of Nodes and Internodes [DOI]
- 21 Some Morphological Aspects of Fruit Tissues Grown In vitro [DOI]
- 22 Morphogenesis in cell cultures of gymnosperms: some growth patterns
- 23 PRODUCTION OF MULTIPLE SHOOTS FROM CAROB TREE (CERATONIA SILIQUA) USING TISSUE CULTURE TECHNIQUE [DOI]
- 24 INDUCTION OF CALLUS FROM HYPOCOTYLEDONS AND COTYLEDONARY LEAVES OF CERATONIA SILIQUA [DOI]
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