Skip to content
Research Article Open access CC BY 4.0

Bioefficacy of Indigenous Isolates of Biocontrol Fungi and Bacteria against Macrophomina Phaseolina Causing Root Rot Disease in Green Gram

Irfan Ahmad, Mujeebur Rahman Khan

Microbiology Research Journal International · pp. 1–10 · Published 17 May 2024

10.9734/mrji/2024/v34i61447

Abstract

Green grams are the most valuable pulse crops in terms of plant-based protein, dietary fiber, and various phytochemicals. Although green gram is found susceptible to the soil-borne fungus Macrophomina phaseolina (Tassi) Goid, it leads to severe root-rot disease and causes a significant reduction in crop yield. Thus, the study aims to determine the bioefficacy of indigenous isolates of biocontrol fungi and bacteria viz., T. asperelloides AMUTASPD-51, T. asperellum AMUTASPM-51, T. asperellum AMUTASPM-52, T. asperellum AMUTASPM-53, T. atroviride AMUTATROV-31, T. harzianum AMUTHZ-71, T. harzianum AMUTHZ-72, T. harzianum AMUTHZ-73, T. harzianum AMUTHZ-74, T. hamatum AMUTHM-31, T. viride AMUTVR-61, T. viride AMUTVR-62, T. virens AMUTVNS-41, T. longibrachiatum AMUTLONB-41, Pochonia chlamydosporia AMUPC-31, Purpureocillium lilacinum AMUPL-31, Aspergillus niger AMUAN-41, Bacillus subtilis AMUBS-80 and Pseudomonas fluorescens AMUPF-80 against Macrophomina phaseolina AMUMP-2 by using dual inoculation technique for seven days incubation at a temperature under in-vitro condition. All species of Trichoderma fungus showed high biocontrol potential to suppress the radial growth of M. phaseolina AMUMP-2 over control. Among the biocontrol fungi and bacteria, T. viride AMUTVR-61 resulted in the highest radial inhibition of M. phaseolina AMUMP-2 by 95.0% over control. The T. harzianum AMUTHZ-72 was second most effective in decreasing the radial growth by 94.2% of the pathogens, followed by T. harzianum AMUTHZ-71 (92.8%), T. asperellum AMUTASPM-53 (86.1%), and T. harzianum AMUTHZ-74 (83.7%) over control. However, B. subtilis AMUBS-80 was found to be the least effective, suppressing radial inhibition of M. phaseolina AMUMP-2 by 21.7% over control. The present study indicates that T. viride AMUTVR-61 and T. harzianum AMUTHZ-72 were the most significant indigenous biocontrol fungi against M. phaseolina AMUMP-2. Furthermore, its application led to a substantial decrease in the soil-borne pathogen population that affects plant health, especially green gram, and adverse environmental and human effects.

Biocontrol agents M. phaseolina AMUMP-2 Trichoderma spp. Aspergillus spp green gram

References (22)

  1. 1 Overview of Mechanisms and Uses of Trichoderma spp. [DOI]
  2. 2 Trichoderma Secondary Metabolites Active on Plants and Fungal Pathogens [DOI]
  3. 3 Biotic and Abiotic Constraints in Mungbean Production—Progress in Genetic Improvement [DOI]
  4. 4 Biology and pathology of Macrophomina phaseolina.
  5. 5 Perspectives and Challenges for Sustainable Management of Fungal Diseases of Mungbean [Vigna radiata (L.) R. Wilczek var. radiata]: A Review [DOI]
  6. 6 Plant growth promoting rhizobacteria and their biopriming for growth promotion in mung bean (Vigna radiata (L.) R. Wilczek) [DOI]
  7. 7 Bioefficacy of Trichoderma Species Against Javanese Root-Knot Nematode, Meloidogyne javanica, in Green Gram [DOI]
  8. 8 Management of root-rot disease complex of mungbean caused by Macrophomina phaseolina and Rhizoctonia solani through soil application of Trichoderma spp. [DOI]
  9. 9 Evaluation of Biocontrol Potential of Seven Indigenous Trichoderma Species against Charcoal Rot Causing Fungus, Macrophomina phaseolina [DOI]
  10. 10 Primary red cell hydration disorders: Pathogenesis and diagnosis [DOI]
  11. 11 In vitro screening of Aspergillus spp. for their biocontrol potential against Macrophomina phaseolina [DOI]
  12. 12 Root–Knot Nematodes in Cereal and Pulse Crops, and Their Management by Novel Biological and Biotechnological Approaches [DOI]
  13. 13 Biological Control of Root-rot on Mungbean Plants Incited By Macrophomina phaseolina Through Microbial Antagonists [DOI]
  14. 14 Integrated management of Macrophomina blight of mungbean (Vigna radiata L.) caused by Macrophomina phaseolina (Tassi) Goid [DOI]
  15. 15 Efficacy of pre- and post-emergence herbicides in rainy season greengram (Vigna radiata) [DOI]
  16. 16 Management of Dry Root Rot [Macrophomina phaseolina (Tassi.) Goid] of Mungbean (Vigna radiata L.) through Bioagents and Bio-fertilizer In vivo [DOI]
  17. 17 Molecular and biochemical characterization of soil isolates of Aspergillus niger aggregate and an assessment of their antagonism against Rhizoctonia solani [DOI]
  18. 18 Recent advancement in management of soybean charcoal rot caused by Macrophomina phaseolina [DOI]
  19. 19 The antagonistic effect of locally isolated Trichoderma spp. against dry root rot of mungbean [DOI]
  20. 20 Isolation and Partial Purification of a Phytotoxin Produced by Macrophomina phaseolina [DOI]
  21. 21 Study on Seed Quality and Performance of Some Mungbean Varieties in Pakistan
  22. 22 Deterioration of seed quality in mung bean by Macrophomina phaseolina (Tassi) Goid. and its management. [DOI]

Cited by 5

Pathogenic diversity and fungicide sensitivity of soybean root rot oomycetes in Heilongjiang Province, China

Zhanyun Liu, Yige Yang, Xueyang Wang · Pest Management Science · 2025

Synergistic interactions of biocontrol agents and chemical fungicides enhance the disease resistance in Vigna radiata (L.) against Macrophomina phaseolina-associated with dry root rot

M. Danish, Mohammad Shahid, M. Farah · Physiological and molecular plant pathology · 2024

Showing 2 of 5 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

71

Page views

0

PDF downloads

23

Outbound clicks

5

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.