Aims: To optimize laccase production by submerged fermentation using an edible mushroom Pleurotus ostreatus ARC280. Study Design: Laccase activity was assayed by monitoring the product formation rate of enzymatic oxidation of syringaldazine spectrophotometrically at 525 nm. Place...
Ali M. Elshafei, Maysa A. Elsayed, Mohamed M. Hassan, Bakry M. Haroun, Abdelmageed M. Othman & Ayman A. Farrag·Annual Research & Review in Biology·2017
Aim: The aim of the present study was to investigate the potential effect of laccase enzyme produced by the fungus Pleurotus ostreatus to decolorize six synthetic dyes in presence and absence of hydroxybenzotriazole (HBT) as a laccase mediator. Study Design: Qualitative and quant...
Open access
Research Article10.9734/ARRB/2017/35644
Aim: the present study aims to optimize Cibacron Blue 3G-A decolorization as a model dye through laccase enzymatic biocatalysis presenting the role of HBT as a redox mediator via RSM approach. Study Design: RSM using Central Composite Design (CCD) was used in order to determin...
Open access
Research Article10.9734/ARRB/2018/40772
Ali M. Elshafei, Abdelmageed M. Othman, Mohamed M. Hassan, Bakry M. Haroun, Maysa A. Elsayed & Ayman A. Farrag·Biotechnology Journal International·2015
Aim: The aim of the present study was to evaluate qualitatively the decolorization of five dyes by Pleurotus ostreatus (P. ostreatus) ARC280 using solid medium. The laccase produced by the fungus was used in terms of its concentration and thermal stability for enzymatic decoloriz...
Open access
Research Article10.9734/BBJ/2015/19505
Aims: To purify, characterize, and apply the laccase produced by submerged fermentation using an edible mushroom Pleurotus ostreatus ARC280. Study Design: Laccase purification and characterization were designed using the most recent approaches and statistical studies of triplica...
Open access
Research Article10.9734/BMRJ/2014/11218