Liming Guan

Plant Regeneration and Its Functional Analysis within Transgenic Rice of ASG-1, an Apomixis-specific Gene Isolated from Apomictic Guinea Grass

Lanzhuang Chen, Liming Guan, Daiki Toyomoto, Toru Sugita, Takuro Hamaguchi & Reiji Okabe · Biotechnology Journal International · 2016

Aims: To analyze the function of the ASG-1, an apomixis-specific gene has been isolated from apomictic guinea grass, the combination of Agrobacterium-mediated transformation system and pSMA35H2-NG binary vector were used for transformation of ASG-1 to ‘Nipponbare’ of rice, and it...

Open access Research Article 10.9734/BJI/2016/29146

Establishment of an Efficient and Practical Virus-free Seedling Supply System by Means of Culture of Shoot Apexes, RT-PCR and Clonal Propagation in Sweet Potato (Ipomoea batatas)

Lanzhuang Chen, Chenti Xu, Zhaosheng Du, Takuro Hamaguchi, Toru Sugita, Ryutarou Nagata & Liming Guan · Biotechnology Journal International · 2013

Aims: Sweet potato (Ipomoea batatas) cv. “Miyazakibeni” was used as material for shoot apex culture, reverse transcription-polymerase chain reaction (RT-PCR) and clonal propagation to establish an efficient and practical virus-free seedling supply system in production of vegetati...

Open access Research Article 10.9734/BBJ/2014/6235

Establishment of Agrobacterium-Mediated Transformation System in Sweet Potato (Ipomoea batatas) by Culture of Leaf Segments for Functional Analysis of ASG-1, an Apomixis-Specific Gene

Lanzhuang Chen, Chengti Xu, Zhaosheng Du, Takuro Hamaguchi, Toru Sugita, Hiroaki Ichikawa & Liming Guan · Biotechnology Journal International · 2013

Aims: In order to do the functional analysis of apomixis-specific gene (ASG-1), which was isolated from apomictic guineagrass, the sweet potato was used to establish an Agrobacterium-mediated transformation system. Study Design: At first, plant regeneration was achieved from the...

Open access Research Article 10.9734/BBJ/2013/4582