Aims: The purpose of this study is to get transformants by using the seeds of sweet potato and the instrument of NEPA21, an electroporation method. Study Design: As the first step, we used grafting method to get seeds from the combinations of Ipomoea crassicaulis cv. [Kidachiasag...
Open access
Research Article10.9734/BJI/2017/35275
Aims: To analyze the function of the ASG-1, an apomixis-specific gene has been isolated from apomictic guinea grass, the combination of Agrobacterium-mediated transformation system and pSMA35H2-NG binary vector were used for transformation of ASG-1 to ‘Nipponbare’ of rice, and it...
Open access
Research Article10.9734/BJI/2016/29146
Aims: Sweet potato (Ipomoea batatas) cv. “Miyazakibeni” was used as material for shoot apex culture, reverse transcription-polymerase chain reaction (RT-PCR) and clonal propagation to establish an efficient and practical virus-free seedling supply system in production of vegetati...
Aims: In order to do the functional analysis of apomixis-specific gene (ASG-1), which was isolated from apomictic guineagrass, the sweet potato was used to establish an Agrobacterium-mediated transformation system. Study Design: At first, plant regeneration was achieved from the...