Higo José Neri da Silva

Design and In silico Validation of Primers for Assessing Gene Expression in the Canonical Inflammatory Pathway

Ian Jhemes Oliveira Sousa, Kerolayne de Melo Nogueira, Higo José Neri da Silva & Ester Miranda Pereira · Advances in Research · 2025

Aims: This study aimed to design and validate primers to investigate the expression of key genes of the canonical inflammation pathway, specifically NEMO (NF-kappaB Essential Modulator), MyD88 and IL-1β, in order to ensure feasibility, specificity and efficiency for use in RT-qPC...