DGGE

Methane Cycling Microbial Communities in Natural and Drained Sites of Taldom Peatland, Moscow Region, Russia

Irina Kravchenko, Anna Kizilova, Ekaterina Menko & Andrey Sirin · Annual Research & Review in Biology · 2014

Aims: Drainage of peatlands is known to decrease overall CH4 emission, but the effect on methane cycling microbes is poorly described. For this reason we aimed to reveal the differences in methanogenic and methanotrophic communities between pristine peatlands and its counterpart...

Open access Research Article 10.9734/ARRB/2015/14978

Comparison of two DNA Extraction Protocols Representing Bacterial Community from Bulk Soil by PCR-DGGE

N. R. Martins, S. R. Passos, G. R. Xavier & F. V. Silva · Microbiology Research Journal International · 2014

Aims: The objective of this work was to investigate the efficiency of DNA extraction from bulk soil using the Britânia® mini mixer combined with phenol extraction to study bacterial communities, from three sampling sites localized in São Gonçalo-RJ, Brazil, comparing it to a comm...

Open access Research Article 10.9734/BMRJ/2015/12628

Culture-Independent Analysis of Bacterial Community Composition during Bioremediation of Crude Oil-Polluted Soil

Chioma Blaise Chikere · Microbiology Research Journal International · 2012

Aim: To use cultivation-independent techniques based on DGGE of PCR-amplified 16S rRNA gene and to evaluate bacterial community composition during bioremediation of crude oil-polluted soil. Study Design: Molecular fingerprints of bacterial populations involved in the active phase...

Open access Research Article 10.9734/BMRJ/2012/1565