cloning

Expression of Epsilon Toxin from Clostridium perfringens Type D in E. coli Rosetta (DE3): Study on a New Vaccine Production

Maryam Adibi, Reza Pilehchian Langroudi & Lida Abdolmohammadi Khiav · Microbiology Research Journal International · 2020

Clostridium perfringens is a Gram-positive anaerobic bacterium which is divided based on major toxins into five types (A-E). C. perfringens type D causes fatal enterotoxaemia (pulpy kidney) in sheep and goats that causes heavy economic losses in domestic animals. However current...

Open access Research Article 10.9734/mrji/2020/v30i930264

Development of a New Bifunctional Fusion Protein of Vaccine Strains Clostridium perfringens Type D and Clostridium septicum Epsilon-alpha Toxin Genes

Marzieh Kamalirousta & Reza Pilehchian · Microbiology Research Journal International · 2018

There are various difficulties regarding of produce and design of a suitable fusion protein, and functionality is the most important of this problem. For producing a functional fusion protein, it is necessary to have a proper suitable host to clone a properly designed fusion gene...

Open access Research Article 10.9734/MRJI/2018/39261