Neuroprotective Activity of Catharanthus roseus Ethanol Extract by Acetylcholinesterase Inhibition and Neurite Outgrowth Studies
Nguyet Thi Doan, Duong Hoang Nguyen, Tru Van Nguyen
Annual Research & Review in Biology · pp. 1–12 · Published 30 Oct 2023
10.9734/arrb/2023/v38i830598Abstract
Aims: To investigate biological activities for neuroprotective effect of Catharanthus roseus. Methodology: Catharanthus roseus was identified using DNA barcoding, utilizing matK, trnH-psbA, and rbcL markers. Additionally, thin-layer chromatography (TLC) method was used to analyze the phytochemistry compounds present in the C. roseus extracts. Moreover, acetylcholinesterase (AChE) inhibition activity was tested using a modified Ellman’s method. Finally, neurite outgrowth activity was determined in rat glial C6 cells treated with varying concentrations of C. roseus extracts. Results: Overall, the plant samples which were collected in Laocai, Vietnam were successfully identified through DNA barcoding regions, using trnH-psbA, matK, and rbcL genes. Phytochemical analysis detected the presence of sterols, terpenoids, flavonoids, polyphenolic in the ethanol extract and its fraction from C. roseus. Additionally, the extracts of C. roseus displayed remarkably high acetylcholinesterase inhibitory activity. Moreover, the ethanol extract of C. roseus shown the most potent neurotrophic activity in a preliminary cell-based screening based on C6 cells neurite outgrowth. Conclusion: These results demonstrate that Catharanthus roseus could be a strong candidate for developing pharmacological drugs to treat neurodegenerative diseases.
Cited by 1
1 citation reported by external sources — individual citing-article records aren't available to list yet.
Related research
- It is Time for More Research into Umbilical Cord Stem Cells as a Potential Treatment in Neurological Diseases — shares topic coverage
- Clinical Study of Neurodegenerative Treatment on Glaucoma by Insulin Intravitreal Injection — shares topic coverage
- Cost Effective Pilot-scale Ajmalicine Production by Catharanthus roseus Cell Suspension Cultures in a 100 Lit Bioreactor — shares topic coverage
- Morphological Anomalies in Somatic Embryo Structure in Catharanthus roseus: Improving Embryo Germination by Amending Plant Growth Regulators, Activated Charcoal and Sucrose Level — shares topic coverage
- Phytochemical Analysis, Antioxidant and Antibacterial Activities of Two Traditionally Used Indian Medicinal Plants — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
1
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.