PCR and Sequencing Base Detection of Gummosis Disease on Citrus aurantifolia Caused by Lasiodiplodia theobromae and Evaluation of Its Antagonisms
Md. Faruk Hasan, Md. Asadul Islam, Biswanath Sikdar
Journal of Advances in Microbiology · pp. 77–90 · Published 2 Apr 2020
10.9734/jamb/2020/v20i330230Abstract
Diplodia gummosis disease caused by Lasiodiplodia theobromae is an economically important postharvest fruit decay that occurs on all types of citrus grown in Bangladesh. The present investigation was conducted to isolate and identify the pathogenic fungus responsible for postharvest diplodia gummosis disease (DGD) of citrus as well as the evaluation of its biological control through microbial antagonists. DGD causing pathogen was identified by physiological, morphological and molecular methods. The pathogenic fungus was isolated from the surface of postharvest lime fruits. The optimum growth of the fungi was observed in maltose, 2% NaCl, 15% sugar and pH 7 containing PDA medium at 35⁰C. PCR products of the internal transcribed spacer (ITS) region of the fungus showed approximately 650 bp size clear band in gel electrophoresis. The amplified region of the fungus showed 99.62% similarities with the sequences of L. theobromae. Artificially inoculation of the fungus in malta, musumbi, sweet orange, lime, and guava fruits showed similar size clear band and typical diplodia gummosis disease symptom. Methanol extracts of Datura metal displayed the highest inhibition (75.25%) against the isolated fungus. Non-pathogenic fungi Trichoderma viride showed the highest antagonistic efficiency followed by Neofusicoccum mangifera against the isolated fungus. The tested soil bacteria did not show significant antagonistic activity against the isolated fungus. Therefore, the DGD of citrus control system should be integrated into the overall citrus postharvest decay control system to reduce all citrus postharvest diseases and to protect fresh citrus fruit values.
Cited by 9
Mohammed Faruk Hasan, Mohammed Asadul Islam, Biswanath Sikdar · F1000Research · 2022
Melisa Belén Rosa Manzano, Mario Rajchenberg, Lucía Molina · Journal of Plant Diseases and Protection · 2025
Preangka S. Briste, Abdul M. Akanda, Md. Abdullahil B. Bhuiyan · Journal of Basic Microbiology · 2022
Moussango Victor Davy, Voundi Olugu Steve, Tchabong Raymond Sammuel · Journal of Fungi · 2025
Mohammed Faruk Hasan, Mohammed Asadul Islam, Biswanath Sikdar · F1000Research · 2020
M E Dwiastuti, T G Aji · IOP Conference Series: Earth and Environmental Science · 2021
Mohammed Faruk Hasan, Mohammed Asadul Islam, Biswanath Sikdar · F1000Research · 2020
Mohammed Faruk Hasan, Mohammed Asadul Islam, Biswanath Sikdar · F1000Research · 2023
Ying Liu, Usman Rasheed, Bin Shan · Molecular Biotechnology · 2025
Related research
- Prevalence of Fusarium Species Associated with Peach Decline in Tunisian Nurseries — shares topic coverage
- Biochemical and Molecular Characterization of Bacterial Wilt Disease of Banana and Evaluation of their Antibiotic Sensitivity — shares topic coverage
- Detection of Sutterella in the Stool of Egyptian Children with Autism Spectrum Disorders — shares topic coverage
- Incidence of Heat-Labile Toxin (LT) Producing Enterotoxigenic Escherichia coli Mediated Diarrheal Disease in Egyptian Children under 5 Years Presenting to Tanta University Hospital — shares topic coverage
- Molecular Characterization and Detection of Infection in Vector Snails of Urinary Schistosomiasis around Erinle and Eko Ende Dams in South West Nigeria — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
9
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.