Efficacy of Dry Pituitary Gland, Wet Pituitary Gland and the Synthetic Hormone Ovaprim on Labeo catla Breeding

Abstract

An induced breeding experiment with Indian major carp, catla (Labeo catla) was conducted to know the efficacies of three different doses of dry Pituitary gland and wet Pituitary gland in comparison to that of ovaprim. Intra- peritoneal injections were given at the base of the pelvic fin with dry PG low dose (DPL), 1 mg/Kg body weight for first dose of female fish and after 6 hours interval second dose (2 mg/Kg of body weight) was administered; at the same time 1 mg/Kg body weight for male. For dry PG medium dose (DPM), 2 mg/Kg body weight for first dose and after six hours interval 4 mg/Kg body weight for female and 2 mg/Kg body weight for male. For dry PG high dose (DPH), 3 mg/Kg body weight for first dose and after 6 hours interval 6 mg/Kg body weight for female and 3 mg/Kg body weight for male fish. Wet PG low dose (WPL), 7 mg/Kg body weight for first dose and after six hours interval 10 mg/Kg body weight for female and 7 mg/Kg body weight for male fish. For wet PG medium dose (WPM), 11 mg/Kg body weight for first dose and after 6 hours interval 15 mg/Kg body weight for female and 11 mg/Kg body weight for male fish. And for wet PG high dose (WPH), 15 mg/Kg body weight for first dose and after 6 hours interval 20 mg/Kg body weight for female and 15 mg/Kg body weight for male fish and ovaprim, 0.25ml/kg and 0.5 ml/Kg of body weight for single dose for male and female respectively. These doses were used for determination of fertilization, hatching and survival rates. The fertilization rates were 83.25 ± 0.85%, 92.01 ± 0.45%, 82.45 ± 1.04%, 83.18 ± 0.68%, 88.59 ± 0.48% and 72.85 ± 0.43% for the doses of DPL, DPM, DPH, WPL, WPM and ovaprim respectively. The hatching rates were 78.58 ± 0.94%, 85.54 ± 0.38%, 74.8 ± 0.22%, 77.89 ± 0.82%, 81.56 ± 0.59% and 71.54 ± 0.48% for the doses of DPL, DPM, DPH, WPL, WPM and ovaprim respectively and the survival rates were 77.24 ± 0.49%, 87.12 ± 0.46%, 73.09 ± 0.42%, 75.8 ± 0.30%, 80.76 ± 0.64% and 69.2 ± 0.77% for the doses of DPL, DPM, DPH, WPL, WPM and ovaprim respectively. Among all the doses of dry and wet PG and the synthetic hormone ovaprim, DPM gave the best result in case of fertilization, hatching and survival rate. So, the induced breeding of catla fish may use dry PG at the dose of 2 mg/Kg body weight as first dose and 4 mg/Kg of body weight as second dose.