Oxidal® Ameliorates the Ty1 Retrotransposition Induced by Methyl Methanesulfonate in Saccharomyces cerevisiae

Abstract

Aim: The aim of the study was to evaluate the potential of Oxidal® to decrease the Ty1 retrotransposition rate in a model system Saccharomyces cerevisiae. Study Design: Saccharomyces cerevisiae cell suspensions were pre-treated with different concentrations Oxidal® and subsequently treated with 16mM methyl methanesulfonate. (MMS) Methodology: The potential of various concentrations Oxidal® was evaluated based on “spot” test and Ty1 retro-transposition test. Results: Data revealed that only 5% Oxidal® possesses some cytotoxic properties. Lack of Ty1 retro-transposition was observed after single treatment with 1, 2.5 and 5% Oxidal® concentrations. On the other hand, all the tested concentrations showed promising results against the standard carcinogen methyl methane sulfonate. The most pronounced anti-carcinogenic and cytoprotective effects were observed after pre-treatment with 2.5% Oxidal®, which could be attributed to the antioxidant properties of the combination of ingredients; methylene blue, salicylic acid and caffeine. Further studies could reveal the exact mechanism of action of the supplement and the role of the antioxidant potential. Conclusion: New data is provided concerning the potential of Oxidal® at low concentrations to protect Saccharomyces cerevisiae cells from MMS-induced Ty1 retro-transposition. The cytoprotective properties of the supplement were also obtained. These results could be considered as a basis for further studies revealing the exact mechanisms of cell protection of the Oxidal®. Additionally, our data could also serve as an important step of the in-depth research of a potential antiviral activity.