Determination of in vitro Anticoagulant Effect of Acacia nilotica Extract on Normal Human Blood Samples

Abstract

Herbal medicines are attractive as therapeutic agents for treating different disease either alone or in combination with chemical drugs. Among these, Acacia nilotica have activities such as antimalarial, antioxidant, antifungal and many other activities. This study aimed to determine the effect of Acacia nilotica on coagulation parameters including whole blood clotting time, clot lysis and clot retraction with different concentrations of aqueous extract of Acacia nilotica. The study was conducted in Al-Zaiem Al–Azhari University among 20 apparently healthy individuals during the period between August to September 2021. Whole blood clotting time was measured for blood specimens after the addition of different concentrations of Acacia nilotica aqueous extract (50%, 75% and 100%). After clotting, clot retraction was evaluated and clot lysis time was measured. The results revealed that; the whole blood clotting time was significantly increased after the addition of the extract in relation to the concentration (P value for 75% = 0.012, and 100% = 0.000). Moreover; clot retraction test results were significantly affected by the addition of various concentrations of the extract (P values < 0.05), while no significant effect was observed in clot lysis time after the addition of various concentrations of A. nilotica extract. The study concluded that the aqueous extract of A. nilotica have an in vitro anticoagulation effect on human blood. This effect is related to the concentration of the extract.