Ovicidal Effect of Tannins-containing Extract of Sesbania grandiflora Leaves against Goat’s Parasitic Nematode Egg

Abstract

Aims: In this study, sesbania (Sesbania grandiflora) leaves were used as an experimental material. Tannins was measured using Folin-Ciocalteu method. The ovicidal effect of this plant leaves was determined against goat’s parasitic nematode egg using egg hatch assay. Study Design: Completely Randomized Design (CRD). Place and Duration of Study: Nematode eggs collection and in vitro trial were conducted at Animal Physiology Laboratory, Faculty of Agriculture of Kasetsart University in Bangkok, Thailand between June 2016 and December 2016. Methodology: Two type of extracts; aqueous and ethanolic extracts were prepared prior to the in vitro trial. Six increasing 2-fold concentration of both extracts; from 0.075 to 2.4 mg/ml were used against mixed sample of nematode’s egg from naturally infected goats. Gridded petri dishes containing a mixture of egg suspension with tested solution; both extracts and control were incubated for 27°C for 24hrs and 48hrs, respectively. At the end of each incubation period, hatched and unhatched eggs were counted and egg hatching inhibition rate was determined. Results: Total tannins in this plant was measured at 1.08% of 95% dry matter (DM). Both extracts showed significant inhibition effect for both incubation periods (P<0.05). Highest concentration used for aqueous and ethanolic extracts showed more than 80% and 60% of hatching inhibition after 48hrs, respectively. Significantly higher inhibition rate was observed for aqueous extract compared to ethanolic extract (P<0.05). For aqueous extract, the maximum inhibition rate was observed at 85.33% for the highest concentration after 48hrs which is almost similar to anthelmintic efficacy of oxfendazole used as positive control (96.67%). Conclusion: This study showed that S. grandiflora leaves can be used as natural anthelmintic for goats since their ovicidal effect against nematode’s egg was scientifically proven.