Effect of Dietary Lysine and DL-Methionine Supplementation in Roasted Soyabean-Based Diet on the Growth Performance and Blood Profiles of Clarias gariepinus

Abstract

This study was designed to investigating the growth performance, hematology, and serum biochemistry of Clarias gariepinus nourished with roasted soyabean based diets with varied inclusion of dietary amino acid. A total of 360 Clarias gariepinus juveniles, aged eight weeks and weighing 19.0±0.4 grams, were randomly assigned to six different diets with varying inclusion levels of lysine and DL-methionine (g/100g). The diets were as follows: RS1 (Control) without lysine or DL-methionine supplementation; RS 2 - 0g + 1g; RS 3 - 0.25g + 0.75g; RS 4 - 0.5g + 0.5g; RS 5 - 0.75g + 0.25g; and RS 6 - 1g + 0g of Lysine and DL-methionine. For 84 days, the fish were fed until they were completely satisfied. Each treatment was in triplicate. Supplementing lysine and methionine in a diet based on roasted soybeans did not significantly (P>0.05) effect on the growth parameter such as final weight, feed conversion ratio, and/or specific growth rate in fish. A significantly higher survival rate was observed in fish on diet RS 5 (85.60±0.60) and was closely followed by fish fed diet RS 3 and RS 4. Fish fed diets RS 4 and RS 5 exhibited significantly higher (P<0.05) packed cell volume (PCV %) and hemoglobin levels. While, White blood cell counts (x10^9/L) varied significantly among the different diets. The addition of supplemental amino acids did not significantly (P>0.05) affect total protein (g/L), and the values ranges from 6.60±0.52 (RS 6) to 7.87±0.55 (RS 4). This study showed that supplementation of lysine and DL-methionine in Roasted Soyabean based diet could improve growth performance of fish with no adverse effect on fish health.