Effect of Biostimulant Formulation of Centella asiatica (L.) Urb. Crude Terpenoid Extract with Addition of Micronutrients on the Growth and Yield of Upland Rice (Oryza sativa L.)

Abstract

Aims: To analyze the effect of biostimulant formulation of Centella asiatica terpenoid extract with the addition of micronutrients on the growth and yield of upland rice (Oryza sativa L.). Study Design:  Experimental design. Place and Duration of Study: Laboratory of Plant Physiology, Department of Biology, Andalas University, in agricultural land, Limau Manis, Padang City, Indonesia  from January until April 2021 Methodology: The experiment was laid out in a factorial randomized block design having nine treatments and replicated thrice. The first factor was the concentration of the terpenoid extract: a0 (0 mg/L); a1 (0.25 mg/L); a2 (0.5 mg/L). The second factor is the composition of the solution of micronutrients: b0 (without micronutrients); b1 (FeSO4 0,01875%, MnSO4 0,01562%, ZnSO4 0,01562%, CuSO4 0,00312%, H3BO3 0,00937%); b2 (FeSO4 0,0375%, MnSO4 0,03125%, ZnSO4 0,03125%, CuSO4 0,00625%, H3BO3 0,01875%). Results: The results showed that the biostimulant formulation of terpenoid extract with the addition of micronutrients affected some parameters of growth and yield of upland rice. The highest average number of tillers was 23.00, it was obtained in treatment a2b2. Treatment a1b2 gave a high average dry weight of 100 grains, namely 2.47g. The dry weight of 100 grains was positively correlated (r = 0.702) with the number of tillers, plant height and chlorophyll b with respectively contributions of 9.36%, 7.35% and 3.82%. Conclusion: The biostimulant formulation of C. asiatica crude terpenoid extract with addition of micronutrients was able to increase the number of tillers and dry weight of 100 grains of upland rice.