Validated Spectrophotometric Method for Determination of Some Benzimidazole Derivatives in Pharmaceutical Formulations Using 1,2-naphthoquinone-4-sulphonate
International Research Journal of Pure and Applied Chemistry · pp. 118–132 · Published 22 Mar 2013
10.9734/IRJPAC/2013/3160Abstract
Aim: To develop and validate a sensitive method for the determination of rapeprazole (RPZ) and omeprazole (OPZ) in bulk and formulations based on nucleophilic substitution reaction of RPZ and OPZ with sodium 1,2-naphthoquinone-4-sulphonate (NQS) in an alkaline medium. Study Design: All variables were studied to optimize the reaction conditions and the reaction mechanism was postulated. Place and Duration of Study: Department of Chemistry, Faculty of Science, Aleppo University, Aleppo, Syria between December 2011 and December 2012. Methodology: The colored products were measured spectrophotometrically at 453 nm using double beam UVD-2960 (Labomed, INC., U.S.A) ultraviolet-visible spectrophotometer with matched 1-cm quartz cells. The reaction time and temperature were 20 min and 25ºC for RPZ and OPZ. As per ICH guidelines, the proposed method was validated. The developed method was successfully applied for the estimation of RPZ and OPZ in tablets and capsules and results were compared statistically with the official methods. Results: The developed method showed a linear Beer's law range from 0.26 to 12.0 and from 0.49 to 12.0 μg mL-1 with limit of detection values of 0.181 and 0.187 μg mL-1 for RPZ and OPZ, respectively. The calculated molar absorptivity values are 7.7×104 and 3.8×104 L mol−1 cm−1 for RPZ-NQS and OPZ-NQS, respectively. The proposed methods were successfully applied to the determination of RPZ and OPZ in formulations and the results tallied well with the label claim. Conclusion: The developed method was linear, sensitive, selective, precise, accurate and robust, being suitable for routine quality control analyses of RPZ and OPZ.
References (38)
- 1 Spectrophotometric Methods of Establishing Empirical Formulas of Colored Complexes in Solution [DOI]
- 2 Comparative study of novel spectrophotometric methods manipulating ratio spectra: An application on pharmaceutical ternary mixture of omeprazole, tinidazole and clarithromycin [DOI]
- 3 Statistics in Analytical Chemistry [DOI]
- 4 Spectrophotometric and chromatographic determination of rabeprazole in presence of its degradation products [DOI]
- 5 Method development and validation for the separation and determination of omeprazole enantiomers in pharmaceutical preparations by capillary electrophoresis [DOI]
- 6 Spectrophotometric determination of dapsone in pharmaceutical products using sodium 1,2-naphthoquinone-4-sulfonic as the chromogenic reagent [DOI]
- 7 The alternating current polarographic behavior and determination of lansoprazole and omeprazole in dosage forms and biological fluids [DOI]
- 8 Spectrophotometric simultaneous determination of Rabeprazole Sodium and Itopride Hydrochloride in capsule dosage form [DOI]
- 9 The Application of Mathematical Statistics to Chemical Analysis [DOI]
- 10 The potency of substituted benzimidazoles such as E3810, omeprazole, RO 18-5364 to inhibit gastric H+,K+-ATPase is correlated with the rate of acid-activation of the inhibitor [DOI]
- 11 Experimental design in the development of voltammetric method for the assay of omeprazole [DOI]
- 12 Nonaqueous capillary electrophoresis for the analysis of labile pharmaceutical compounds [DOI]
- 13 Omeprazole determination using HPLC with coulometric detection [DOI]
- 14 High-performance liquid chromatography method for the quantification of rabeprazole in human plasma using solid-phase extraction [DOI]
- 15 Quantification of rabeprazole in human plasma by liquid chromatography–tandem mass spectrometry [DOI]
- 16 Spectrophotometric study for the reaction between fluvoxamine and 1,2-naphthoquinone-4-sulphonate: Kinetic, mechanism and use for determination of fluvoxamine in its dosage forms [DOI]
- 17 Development of a liquid chromatography/tandem mass spectrometry assay for the quantification of rabeprazole in human plasma [DOI]
- 18 HPLC Analysis for Simultaneous Determination of Rabeprazole and Domperidone in Pharmaceutical Formulation [DOI]
- 19 New Liquid Chromatographic Method for Determination of Rabeprazole Sodium in Coated Tablets [DOI]
- 20 Spectrophotometric determination of certain benzimidazole proton pump inhibitors [DOI]
- 21 Enantiospecific resolution of rabeprazole by liquid chromatography on amylose-derived chiral stationary phase using photo diode array and polarimetric detectors in series [DOI]
- 22 Quantitative Analysis of Rabeprazole Sodium in Commercial Dosage Forms by Spectrophotometry [DOI]
- 23 HPTLC Determination of Rabeprazole and Domperidone in Capsules and its Validation [DOI]
- 24 Simultaneous Spectrophotometric Estimation of Rabeprazole Sodium and Itopride HCl [DOI]
- 25 Spectrophotometric method for estimation of rabeprazole [DOI]
- 26 Determination of iron(III) and copper(II) by zeroth, first and second derivative spectrophotometry with 2-thiobarbituric acid (4,6-dihydroxy-2-mercaptopyrimidine) as reagent [DOI]
- 27 Chiral separation of omeprazole and several related benzimidazoles using supercritical fluid chromatography [DOI]
- 28 Voltammetric behaviour of rabeprazole at a glassy carbon electrode and its determination in tablet dosage form [DOI]
- 29 Simple quantification of lansoprazole and rabeprazole concentrations in human serum by liquid chromatography/tandem mass spectrometry [DOI]
- 30 Development and Validation of Derivative Spectrophotometric Method for Determination of Rabeprazole Sodium in Pharmaceutical Formulation [DOI]
- 31 High-Performance Liquid Chromatography and Thin-Layer Chromatography for the Simultaneous Quantitation of Rabeprazole and Mosapride in Pharmaceutical Products [DOI]
- 32 Column Reversed-Phase High-Performance Liquid Chromatographic Method for Simultaneous Determination of Rabeprazole Sodium and Domperidone in Combined Tablet Dosage Form [DOI]
- 33 High-performance thin-layer chromatographic determination of rabeprazole sodium and domperidone in combined dosage form.
- 34 Simultaneous spectrophotometric estimation of rabeprazole sodium and itopride hydrochloride in capsule formulations [DOI]
- 35 Rabeprazole for the prevention of pathologic and symptomatic relapse of erosive or ulcerative gastroesophageal reflux disease [DOI]
- 36 Validation of a Capillary Electrophoresis Method for Analysis of Rabeprazole Sodium in a Pharmaceutical Dosage Form [DOI]
- 37 Spectrophotometric and chromatographic determination of omeprazole in pharmaceutical formulations [DOI]
- 38 Simultaneous Estimation Of Amoxycillin Trihydrate And Rabeprazole Sodium
Cited by 10
Department of Medicinal Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt, Samar S. Elbaramawi, Mohamed E. El-Sadek · Chemical Reports · 2020
Abdalla Ahmed Elbashir, Iman Ahmed Alamin · Journal of Analytical & Pharmaceutical Research · 2019
Safwan Ashour · Chinese Journal of Analytical Chemistry · 2025
Mohamed I. Walash, Fawzia Ibrahim, Samah Abo El Abass · Analytical Methods · 2013
Showing 4 of 10 known citations — external sources report more than can currently be individually listed.
Related research
- Expert Opinion on the Prescription Practice of Rabeprazole and Other Common Proton Pump Inhibitors for Patients with Gastroesophageal Reflux Disease — shares topic coverage
- Expert Opinion on the Prescription Practice of Proton Pump Inhibitor for the Treatment of Gastroesophageal Reflux Disease — shares topic coverage
- A Comparative Evaluation of Lafutidine and Rabeprazole in the Treatment of Gastritis and Peptic Ulcer: A Double-blind, Randomized Study in Indian Patients — shares topic coverage
- Anti-ulcer Mechanisms of L-lysine in Male Wistar Rats — shares topic coverage
- Upper Gastrointestinal Bleeding in the Absence of Proper pH Control in Patients Admitted to ICU — shares topic coverage
Article metrics
Real usage data collected on this platform.
72
Page views
0
PDF downloads
25
Outbound clicks
10
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.