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Update of Regulatory Options of New Breeding Techniques and Biosafety Approaches among Selected Countries: A Review

Silas Obukosia, Olalekan Akinbo, Woldeyesus Sinebo, Moussa Savadogo, Samuel Timpo, Modupe Adeyemo, Sunday Akile, Jean Kebere, Jeremy Ouedraogo, Aggrey Ambali, Diran Makinde

Asian Journal of Biotechnology and Bioresource Technology · pp. 18–35 · Published 17 Nov 2020

10.9734/ajb2t/2020/v6i330083

Abstract

A new set of breeding techniques, referred to as New Breeding Techniques developed in the last two decades have potential for enhancing improved productivity in crop and animal breeding globally. These include site directed nucleases based genomic editing procedures-CRISPR and Cas associated proteins, Zinc Finger Nucleases, Meganucleases/Homing Endonucleases and Transcription- Activator Like-Effector Nucleases for genome editing and other technologies including- Oligonucleotide-Directed Mutagenesis, Cisgenesis and intragenesis, RNA-Dependent DNA methylation; Transgrafting, Agroinfiltration, Reverse breeding. There are ongoing global debates on whether the processes of and products emerging from these technologies should be regulated as genetically modified organisms or approved as conventional products. Decisions on whether to regulate as GMOs are based both on understanding of the molecular basis of their development and if the GMO intermediate step was used. For example- cisgenesis, can be developed using Agrobacterium tumefaciens methods of transformation, a process used by GMO but if the selection is properly conducted the intermediate GMO elements will be eliminated and the final product will be identical to the conventionally developed crops. Others like Site Directed Nuclease 3 are regulated as GMOs in countries such as United State of America, Canada, European Union, Argentina, Australia. Progress in genome editing research, testing of genome edited bacterial blight resistant rice, development of Guidelines for regulating new breeding techniques or genome editing in Africa is also covered with special reference to South Africa, Kenya and Nigeria. Science- and evidence-based approach to regulation of new breeding techniques among regulators and policy makers should be strongly supported.

New breeding techniques regulation USA Canada Australia EU Argentina Africa.

References (38)

  1. 1 CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes [DOI]
  2. 2 Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage [DOI]
  3. 3 Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System [DOI]
  4. 4 Search-and-replace genome editing without double-strand breaks or donor DNA [DOI]
  5. 5 Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage [DOI]
  6. 6 Xanthomonas AvrBs3 Family-Type III Effectors: Discovery and Function [DOI]
  7. 7 RNA-directed de novo methylation of genomic sequences in plants [DOI]
  8. 8 Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA [DOI]
  9. 9 Broad-spectrum resistance to bacterial blight in rice using genome editing [DOI]
  10. 10 Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity [DOI]
  11. 11 Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems [DOI]
  12. 12 In vivo analysis of plant promoters and transcription factors by agroinfiltration of tobacco leaves [DOI]
  13. 13 Homing endonucleases: keeping the house in order [DOI]
  14. 14 CRISPR-Cas12a: Functional overview and applications [DOI]
  15. 15 Plant Genome Engineering with Sequence-Specific Nucleases [DOI]
  16. 16 Applications and potential of genome editing in crop improvement [DOI]
  17. 17 Dual base editor catalyzes both cytosine and adenine base conversions in human cells [DOI]
  18. 18 A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing [DOI]
  19. 19 Heritable targeted mutagenesis in maize using a designed endonuclease [DOI]
  20. 20 Targets of RNA-directed DNA methylation [DOI]
  21. 21 Site-directed nucleases: a paradigm shift in predictable, knowledge-based plant breeding [DOI]
  22. 22 Gene targeting in plants: 25 years later [DOI]
  23. 23 Oligonucleotide‐directed mutagenesis for precision gene editing [DOI]
  24. 24 A future scenario of the global regulatory landscape regarding genome-edited crops [DOI]
  25. 25 Genome editing for horticultural crop improvement [DOI]
  26. 26 What Is Epigenetics? [DOI]
  27. 27 Gene Delivery into Plant Cells for Recombinant Protein Production [DOI]
  28. 28 Genome modifications in plant cells by custom‐made restriction enzymes [DOI]
  29. 29 Targeted gene correction of episomal DNA in mammalian cells mediated by a chimeric RNA.DNA oligonucleotide. [DOI]
  30. 30 A tool for functional plant genomics: Chimeric RNA/DNA oligonucleotides cause in vivo gene-specific mutations [DOI]
  31. 31 Targeted manipulation of maize genes in vivo using chimeric RNA/DNA oligonucleotides [DOI]
  32. 32 Transformation of yeast with synthetic oligonucleotides. [DOI]
  33. 33 Targeted DNA excision in Arabidopsis by a re-engineered homing endonuclease [DOI]
  34. 34 Two Unlinked Double-Strand Breaks Can Induce Reciprocal Exchanges in Plant Genomes via Homologous Recombination and Nonhomologous End Joining [DOI]
  35. 35 Oligo-Mediated Targeted Gene Editing [DOI]
  36. 36 Current status on the modification of the scope for GMO regulation on the gene edited plants with no remnants of inserted foreign DNA fragments [DOI]
  37. 37 Targeted genome modification of crop plants using a CRISPR-Cas system
  38. 38 The Genesis Engine

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