The Effect of Long-Term Vitamin E Therapy on Hepatotoxic Injury in Male Wistar Rats

Abstract

PQ (1, 1-dimethyl-4, 4-bipyridilium dichloride) is a commercial herbicide that increases agricultural yield by killing weeds all over the world. Vitamin E (-tocopherol) is a fat-soluble antioxidant found in all cellular membranes.It helps to protect the cell from lipid peroxidation. The purpose of this study was to see how Vitamin E affected paraquat-induced liver damage in rats. For the study, 200 male albino rats were used. The rats were divided into four groups of 50 rats each (A, B, C, D) and then subdivided into 25 rats per subgroup. The "A" group received no paraquat, whereas the "B," "C," and "D" groups received increasing doses of 0.02 g, 0.04 g, and 0.06 g, respectively. The "A" group was divided into two subgroups: "Ao" and "Ave," which were the subgroups that were not treated with Vit E and the subgroup that was treated with Vit E (500 mg), respectively. This was also true for groups "B", "C", and "D". For three months, paraquat was given every fourth night, followed by weekly vitamin E treatments. Blood was drawn and tested for liver function (total and direct bilirubin, total protein, albumin, and globulin), and the liver was harvested for histological examination. There was a significant difference in the level of all parameters between the "Ao," "Bo," "Co," and "Do," p-value0.05, as well as between the "AVEC," "BVEC," "CVEC," and "DVEC," p-value <0.05.The results also demonstrated that while there was no significant difference between the AO and AVE subgroups for any of the liver biochemical parameters, there were significant differences in intra-group comparisons for all of the liver biochemical parameters, with p-values<0.05. Additionally, there was no discernible variation in globulin concentration between the groupings. This study has proven that weekly treatment for three months with vitamin E has a therapeutic impact on male rats. Therefore, a weekly vitamin E treatment can lessen some of the liver damage paraquat induced in rats.