Detection of Resistance Integron in Escherichia coli of Porcine Origin Producing Extended-spectrum Beta-lactamase in Abidjan, Côte d'Ivoire

Abstract

Aims: Aim of the study was to detect resistance integrons involved in multidrug resistance phenotypes in Escherichia coli strains of porcine origin producing extended-spectrum beta-lactamases (ESBL). Study Design: Genotypic study. Place and Duration of Study: National Reference Center for Antibiotics and Molecular Biology Platform of Pasteur Institute of Côte d'Ivoire, between June 2017 at July 2017. Methodology: Thirty-five (35) Escherichia coli strains of porcine origin producing extended-spectrum beta-lactamases were collected for study. The strains were analyzed using an antibiotic susceptibility test according to the diffusion method in agar medium. The research of class 1, 2 and 3 resistance integrons was performed using the conventional PCR method. Results: 25 strains (71%) of E. coli producing ESBL harboured class 1 resistance integrons. None of the isolates carried class 2 and 3 resistance integrons. The strains harbouring resistance integrons were more resistant to amoxicillin, ceftriaxone, cefotaxime, tetracycline with a much higher rate of resistance (71%) compared to integron negative isolates (31%). The resistance to kanamycin and cotrimoxazole were 60% in integron positive isolates. Concerning integron negative isolates, the resistance to kanamycin was 11% and cotrimoxazole 20%.   Conclusion: The integron positive isolates is one of the major causes of resistance gene dissemination. This represents a risk for public health that must challenge the public authorities on the reasoned use of antibiotics in animal production chains.