Trans Sialidase Genes Allow Clustering of TcI Trypanosoma cruzi Mexican Isolates
V. M. Monteón, R. López, A. Ramos-Ligonio, K. Acosta-Viana
Microbiology Research Journal International · pp. 1299–1310 · Published 20 Jul 2014
10.9734/BMRJ/2014/11060Abstract
Aims: Polymorphism of the trans sialidase (TS) family of genes is common in Trypanosoma cruzi. Our goal was to cluster Mexican TcI DTU (Discrete Typing Unit) using a set of primers specific for TS genes. Methodology: The DNA of 12 Mexican T. cruzi stocks (TcI) and reference strain were amplified using the CRP-1 primer, which anneals to the conserved 5' ends of CRP (Complement Regulatory Protein), TS, and FL-160 genes, and the CRP-2 primer, which anneals to conserved region within the GPI (Glycosil Phosphatidil Inositol) anchor sequence. Amplicons were analysed using PCR-SSCP (Single Strand Conformation Polymorphims) followed by construction of a nominal matrix data (presence/absence bands) to calculated the Jaccard and Dice similarity coefficient, and clustering with UPGMA. Results: Mexican TcI stocks produced a common pattern of amplification products and cluster in a separate group to CL-Brener strain (TcVI). The PCR-SSCP revealed that within the TcI Mexican stocks there were a complex pattern, but T. cruzi from the Yucatan peninsula clustered in special and separate group. Conclusion: The CRP-1 and CRP-2 primers were helpful for the analysis of genetic traits in T. cruzi DTU I and revealed the existence of special group in Yucatan Mexico.
References (19)
- 1 The revised Trypanosoma cruzi subspecific nomenclature: Rationale, epidemiological relevance and research applications [DOI]
- 2 Genomic Analyses, Gene Expression and Antigenic Profile of the Trans-Sialidase Superfamily of Trypanosoma cruzi Reveal an Undetected Level of Complexity [DOI]
- 3 Haplotype identification within Trypanosoma cruzi I in Colombian isolates from several reservoirs, vectors and humans [DOI]
- 4 Sequence properties of GPI-anchored proteins near the omega-site: constraints for the polypeptide binding site of the putative transamidase [DOI]
- 5 Structural Characterization of the Major Glycosylphosphatidylinositol Membrane-anchored Glycoprotein from Epimastigote Forms of Trypanosoma cruzi Y-strain [DOI]
- 6 GPIomics: global analysis of glycosylphosphatidylinositol‐anchored molecules of Trypanosoma cruzi [DOI]
- 7 Identification by Q-PCR of Trypanosoma cruzi lineage and determination of blood meal sources in triatomine gut samples in México [DOI]
- 8 Differential Distribution of Genes Encoding the Virulence Factor Trans-Sialidase along Trypanosoma cruzi Discrete Typing Units [DOI]
- 9 Enzymically inactive members of the trans-sialidase family from Trypanosoma cruzi display -galactose binding activity [DOI]
- 10 Identification of the gene family encoding the 160-kilodalton Trypanosoma cruzi complement regulatory protein [DOI]
- 11 Microsatellite and Mini-Exon Analysis of Mexican Human DTU I Trypanosoma cruzi Strains and Their Susceptibility to Nifurtimox and Benznidazole [DOI]
- 12 High Frequency of Human Blood in Triatoma dimidiata Captured Inside Dwellings in a Rural Community in the Yucatan Peninsula, Mexico, but Low Antibody Seroprevalence and Electrocardiographic Findings Compatible with Chagas Disease in Humans [DOI]
- 13 Genetic Structure and Expression of the Surface Glycoprotein GP82, the Main Adhesin of Trypanosoma cruzi Metacyclic Trypomastigotes [DOI]
- 14 The Trypanosoma cruzi neuraminidase contains sequences similar to bacterial neuraminidases, YWTD repeats of the low density lipoprotein receptor, and type III modules of fibronectin. [DOI]
- 15 Sequence Diversity of theTrypanosoma cruziComplement Regulatory Protein Family [DOI]
- 16 Conserved Regions as Markers of Different Patterns of Expression and Distribution of the Mucin-Associated Surface Proteins of Trypanosoma cruzi [DOI]
- 17 Characterisation of Trypanosoma cruzi populations by dna polymorphism of the cruzipain gene detected by single-stranded dna conformation polymorphism (SSCP) and direct sequencingfn1fn1Note: The novel nucleotide sequence data reported in this paper are available in the embl, GenBankTM and DDBL databases under the accession numbers AB006742, AB006743 and AB006744. [DOI]
- 18 Identification of Trypanosoma cruzi sublineages by the simple method of single-stranded conformation DNA polymorphism (SSCP) [DOI]
- 19 Exploring the FL-160-CRP gene family through sequence variability of the complement regulatory protein (CRP) expressed by the trypomastigote stage of Trypanosoma cruzi [DOI]
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