Therapeutic Efficacy of Autologous Platelet-Rich Plasma in Bilateral Corneal Ulcers in a Canine Patient: A Pilot Clinical Study on Ocular Surface Regeneration

Abstract

Background and Aims: Corneal ulcers are among the most frequently encountered ophthalmic conditions in small animal practice, particularly in brachycephalic breeds that are predisposed to corneal exposure and trauma. Delayed epithelial healing and recurrence are common challenges associated with conventional therapy. The present study aimed to evaluate the therapeutic efficacy of autologous platelet-rich plasma (PRP) eye drops in the management of bilateral corneal ulcers in a canine patient and to assess its regenerative potential in ocular surface healing. Study Design: Pilot clinical observational study. Place and Duration of Study: The study was conducted at Sanchu Animal Hospital, Chennai, India, with a follow-up period of one month. Methodology: A 4-month-old female Shih Tzu puppy weighing 2.5 kg was presented with bilateral corneal ulcers characterized by blepharospasm, epiphora, and corneal opacity. Initial treatment with systemic antibiotics (ceftriaxone–tazobactam) and topical antimicrobial and lubricating agents failed to produce clinical improvement. Autologous PRP was prepared by centrifugation of whole blood at 1200 rpm for 10 minutes and administered topically four times daily for a period of 20 days. Clinical parameters including ulcer size, fluorescein staining, corneal opacity grading, and pain scoring were monitored on days 0, 10, 15, and 30. Results: Initial ulcer size measured 4.2 mm (right eye) and 3.8 mm (left eye). Significant reduction in ulcer size was observed by day 10 (1.2 mm and 1.0 mm, respectively), with negative fluorescein staining indicating epithelial healing. Complete epithelialization was achieved by day 15. Pain scores decreased from severe (3) to none (0), and corneal opacity improved from grade 3 to grade 1 by day 30. No recurrence was observed during follow-up. Conclusion: Autologous PRP therapy significantly accelerated corneal healing and improved clinical outcomes in a canine patient with bilateral corneal ulcers. PRP may serve as a safe, effective, and regenerative therapeutic adjunct in veterinary ophthalmology, particularly in refractory cases.