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Research Article Open access CC BY 4.0

The Synergistic Effects of Humic Substances and Biofertilizers on Plant Development and Microbial Activity: A Review

Jose Franco Da Cunha Leme Filho, Wade E. Thomason, Gregory K. Evanylo, Xunzhong Zhang, Michael S. Strickland, Bee K. Chim, Andre A. Diatta

International Journal of Plant & Soil Science · pp. 56–75 · Published 24 Jun 2020

10.9734/ijpss/2020/v32i730306

Abstract

Agroecosystem and ecological cycling loops are open when considering the reutilization of inputs applied in farming areas. Non-renewable resources have been transformed or relocated from the air, water and land into the system and are flowing out as wastes rather than reusable, recyclable resources. This current environmental situation is promoting the development of methods able to optimize nutrient cycling, minimize use of external inputs, and maximize input use efficiency. Some humic products are derived from lignin found in wheat straw and biofertilizers as compost and manure teas can be made using residues. Also, these biostimulants might decrease the necessity of synthetic inputs. This review strives to enhance our understanding of the conjunctive use of humic substances (HS) and biofertilizers. The biostimulant effects of each of these compounds are shown in the literature. Thus, our review question is whether the combined application of HS and biofertilizers can promote synergy between both compounds and potentially more efficacy. The effects promoted by using HS plus biofertilizers on plants and microorganisms are very interconnected, so sometimes these effects can be confounded. For instance, the root elongation promoted by HS might increase hyphal fungi colonization. Therefore, this review as divided in three sections: Responses of plants, fungi and bacteria. The findings indicate that the source and application rate of HS will have a strong impact on whether plant growth and microbial activity significantly improved. The microbial species and plant type also influence the response to HS. The prospects of the conjunctive use of and biofertilizers to stimulate plant development and microbial activity in agricultural systems are theoretically substantial when considering the total number of studies included in this review.

Biostimulants humic acid humate plant growth compost tea manure tea.

References (50)

  1. 1 The contentious nature of soil organic matter [DOI]
  2. 2 Plant growth promoting rhizobacteria as biofertilizers [DOI]
  3. 3 Phosphate solubilizing bacteria and their role in plant growth promotion [DOI]
  4. 4 Trichoderma virens , a Plant Beneficial Fungus, Enhances Biomass Production and Promotes Lateral Root Growth through an Auxin-Dependent Mechanism in Arabidopsis [DOI]
  5. 5 Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial [DOI]
  6. 6 The supramolecular structure of humic substances: A novel understanding of humus chemistry and implications in soil science [DOI]
  7. 7 Physiological responses to humic substances as plant growth promoter [DOI]
  8. 8 Plant nutrition for food security : a guide for integrated nutrient management
  9. 9 THE PRINCIPLES OF HUMIC SUBSTANCES [DOI]
  10. 10 Biological nitrogen fixation: Investments, expectations and actual contributions to agriculture [DOI]
  11. 11 A contribution to set a legal framework for biofertilisers [DOI]
  12. 12 After the Green Revolution [DOI]
  13. 13 Impact of organic manures with and without mineral fertilizers on soil chemical and biological properties under tropical conditions [DOI]
  14. 14 Chemical composition and bioactivity properties of size-fractions separated from a vermicompost humic acid [DOI]
  15. 15 Encapsulation of plant growth‐promoting bacteria in alginate beads enriched with humic acid [DOI]
  16. 16 Molecular Cloning and Regulation of a Mineral Phosphate Solubilizing Gene from Erwinia Herbicola [DOI]
  17. 17 Effect of Aerated Compost Tea on the Growth Promotion of Lettuce, Soybean, and Sweet Corn in Organic Cultivation [DOI]
  18. 18 Closing the nutrient cycle by using bio-digestion waste derivatives as synthetic fertilizer substitutes: A field experiment [DOI]
  19. 19 Probing the hormonal activity of fractionated molecular humic components in tomato auxin mutants [DOI]
  20. 20 Nitrogen-fixing bacteria in nonleguminous crop plants
  21. 21 Organic matter components, aggregate stability and biological activity in a horticultural soil fertilized with different rates of two sewage sludges during ten years [DOI]
  22. 22 PLANT GROWTH REGULATOR ACTIVITY OF SOLUBLE HUMIC COMPLEXES [DOI]
  23. 23 Arbuscular mycorrhizal fungi: a specialised niche for rhizospheric and endocellular bacteria [DOI]
  24. 24 Hyphal growth and mycorrhiza formation by the arbuscular mycorrhizal fungus Glomus claroideum BEG 23 is stimulated by humic substances [DOI]
  25. 25 Rhizosphere microbial diversity as influenced by humic substance amendments and chemical composition of rhizodeposits [DOI]
  26. 26 Effects of a humic acid and its size-fractions on the bacterial community of soil rhizosphere under maize (Zea mays L.) [DOI]
  27. 27 Biological control of Pythium damping-off of pea and sugar beet by Rhizobium leguminosarum bv. viceae [DOI]
  28. 28 Soil fertility management research for the maize cropping systems of smallholders in Southern Africa: A review
  29. 29 Integrated Nutrient Management for Sustainable Crop Production [DOI]
  30. 30 Carbon deposition in soil rhizosphere following amendments with compost and its soluble fractions, as evaluated by combined soil–plant rhizobox and reporter gene systems [DOI]
  31. 31 Effect of Biofertilizer and Humic Acid Applications on Growth, Yield, Quality and Storability of Two Garlic (Allium sativum L.) Cultivars [DOI]
  32. 32 Mechanism of humic acid colour removal from natural waters by fungal biomass biosorption [DOI]
  33. 33 Characteristics of soil humic acid fractions separated by ultrafiltration [DOI]
  34. 34 A possible mechanism for humic acid action on cell elongation in root segments of Pisum sativum under aseptic conditions [DOI]
  35. 35 Influence of humic acids on laurel growth, associated rhizospheric microorganisms, and mycorrhizal fungi [DOI]
  36. 36 Preparation and characterization of model humic polymers containing organic phosphorus [DOI]
  37. 37 Backseat driving? Accessing phosphate beyond the rhizosphere-depletion zone [DOI]
  38. 38 The Effects of Sulfur and Humic Acid on Yield Components and Macronutrient Contents of Spinach (Spinacia Oleracea Var. Spinoza) [DOI]
  39. 39 Vermicompost, plant growth promoting bacteria and humic acid can affect the growth and essence of basil (OcimumbasilicumL.)
  40. 40 Effects of micro-organisms in the transformation of lignin to humic substances [DOI]
  41. 41 Biodegradation of Natural and Synthetic Humic Acids by the White Rot Fungus Phanerochaete chrysosporium [DOI]
  42. 42 Effect of humic acids on numbers and activities of micro-organisms within physiological groups [DOI]
  43. 43 Effect of Soil Salinity on Decomposition and Humification of Organic Matter by Some Cellulolytic Fungi [DOI]
  44. 44 Effects of the extraction temperature on the characteristics of a humic fertilizer obtained from lignite [DOI]
  45. 45 EFFECT OF NITROGEN, HUMIC ACID AND BIO-FERTILIZATION ON PRODUCTIVITY AND QUALITY OF FABA BEAN UNDER SALINE CONDITION [DOI]
  46. 46 Decomposition of Humic Acid by Fungi [DOI]
  47. 47 Influence of sodium humate on the crop plants inoculated with bacteria of agricultural importance [DOI]
  48. 48 Effects of applying humic acids and bio-fertilizer on the qualities and yields of strawberry and soil agrochemical characters.
  49. 49 Use Of Trichoderma Spp. To Improve Plant Performance Under Abiotic Stresses
  50. 50 EVALUATION OF COMPOST TOPDRESSING, COMPOST TEA AND CULTIVATION ON TALL FESCUE QUALITY, SOIL PHYSICAL PROPERTIES AND SOIL MICROBIAL ACTIVITY [DOI]

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