The Performance of L-Theanine as an Immunosimulant to Reduce Stress Levels in Motivant Fish (Helostoma temminkii)

Abstract

Farmed fish that are stressed are susceptible to various diseases that will affect their health, thereby reducing their reproductive abilities. Immunostimulants are substances that can enhance or stimulate the fish's immune system by interacting directly with cells that activate the immune system. The purpose of this study was to analyze the performance of L-theanine as an immunostimulant to reduce stress levels in monkfish. This research was conducted at the Wet Laboratory of the Faculty of Fisheries and Maritime Affairs, Lambung Mangkurat University, South Kalimantan Province. The treatment that will be given is as follows, namely Treatment A with feed added at a dose of L-Theanine 0 mg/kg. Treatment B feed was added with a dose of L-Theanine 300 mg/Kg. Treatment C was supplemented with a dose of L-Theanine 600 mg/Kg. Treatment D was supplemented with a dose of L-Theanine 900 mg/kg. The observed haematological conditions were hematocrit, erythrocytes and blood glucose. The addition of feed with a dose of  L-Theanine 300 mg/kg was able to maintain immunostimulants to reduce stress levels in animal fish according to hematological measurements in the form of hematocrit, erythrocytes and blood glucose.