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Research Article Open access CC BY 4.0

The Effects of the N-hexane Fractions of Heinsia crinita Leaf Extract on Electrolyte Balance and Some Haematological Parameters of Alloxan-induced Diabetic Albino Wistar Rats

Bob I. A. Mgbeje, Esuabanga M. Asenye, Iwara A. Iwara, Godwin O. Igile

Journal of Complementary and Alternative Medical Research · pp. 1–10 · Published 27 Mar 2019

10.9734/jocamr/2019/v7i130094

Abstract

Aim: Crude extracts of Heinsia crinita leaves have been found to have antidiabetic activity. The aim of this study was to evaluate the effects of the n-hexane fraction of Heinsia crinita leaf extract on electrolyte balance and some haematological indices of alloxan-induced diabetic albino Wistar rats. Methodology: Thirty (30) albino Wistar rats of both sexes weighing 120-180g were shared into five (5) groups of six animals each. Group 1 and 2 served as the normal control (NC) and diabetic control (DC) respectively and received placebo. Groups 3, 4 and 5 were diabetic treated group, and received 500mg/kg bw Metformin, 400mg/kg bw of crude extract (HC-C) and n-hexane fraction (HC-H) of Heinsia crinita respectively. Results: The HC-H administered group, like the HC-C group, showed reversal of the observed increase in chloride ion (Cl-) and decrease in sodium ion (Na+) levels in DC group to close to those of the NC group and this effect compared well with the metformin–treated group. The HC-H group, unlike the HC-C group, did not show a reversal of the observed diabetic increase in in potassium ion (K+). The WBC count was increased in DC compared to NC. On treatment with HC–C, HC-H and metformin, the WBC count decreased significantly (P<0.05) in all the treated groups compared to DC group. The RBC count was significantly decreased (P<0.05) in DC compared to NC. Similarly, there was a decrease in haemoglobin concentration in the DC group compared to the NC group. These decreases were significantly (p<0.05) reversed towards normal on treatment with HC-C and HC-H. The reversal was comparable to that of the standard drug metformin. Similarly the diabetes induced increase in platelet counts was significantly (P<0.05) reduced towards NC values on treatment with HC-H and HC-C. Conclusion: The result show that the n-hexane fraction (HC-H), like the crude extract (HC-C), can protect against diabetes induced electrolyte imbalance and haematological disorders.

Diabetes electrolyte balance haematological indices n-hexane fraction Heinsia crinita.

References (22)

  1. 1 The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas [DOI]
  2. 2 The mechanisms of alloxan- and streptozotocin-induced diabetes [DOI]
  3. 3 Chronic Oxidative Stress as a Central Mechanism for Glucose Toxicity in Pancreatic Islet Beta Cells in Diabetes [DOI]
  4. 4 Platelet Dysfunction in Type 2 Diabetes [DOI]
  5. 5 Consequences of the diabetic status on the oxidant/antioxidant balance.
  6. 6 Diabetes mellitus and electrolyte disorders [DOI]
  7. 7 Ethnobotanical studies of medicinal plants used in the management of diabetes mellitus in South Western Nigeria [DOI]
  8. 8 Electrolyte and Acid–Base Disturbances in Patients with Diabetes Mellitus [DOI]
  9. 9 White Blood Cell Count Is Associated With Macro- and Microvascular Complications in Chinese Patients With Type 2 Diabetes [DOI]
  10. 10 Oxidative stress in diabetes mellitus [DOI]
  11. 11 Elevated White Blood Cell Count in Subjects With Impaired Glucose Tolerance [DOI]
  12. 12 Hyperkalemia in diabetes mellitus [DOI]
  13. 13 Erythropoietin and diabetes mellitus [DOI]
  14. 14 Free radicals in biology and medicine (2nd Edition) [DOI]
  15. 15 Erythropoietin depletion and anaemia in diabetes mellitus [DOI]
  16. 16 Membrane Fluidity Is Related to the Extent of Glycation of Proteins, but not to Alterations in the Cholesterol to Phospholipid Molar Ratio in Isolated Platelet Membranes from Diabetic and Control Subjects [DOI]
  17. 17 Some Haematological Parameters in Patients with Type-1 Diabetes in Sokoto, North Western Nigeria [DOI]
  18. 18 Platelet insulin receptor determination in non-insulin dependent diabetes mellitus [DOI]
  19. 19 Evaluation of Antidiabetic and the Effect of Methanolic Leaf Extract of Jatropha curcas on Some Biochemical Parameters in Alloxan-induced Diabetic Male Albino Rats [DOI]
  20. 20 Effect of Phytochemical Fractions of Vernonia amygdalina on Liver of STZ Induced Diabetic Male Wistar Rats [DOI]
  21. 21 Dyselectrolytemia in hyperglycaemic crisis patients with uncontrolled non-insulin dependent diabetes mellitus [DOI]
  22. 22 Biological Effect Of Aqueous Extract Of Heinsia Crinita On Lipid Peroxidation And Angiotensin-1-Converting Enzyme In Vitro [DOI]

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