The Effect of Colchicine on Profiles and Contents of Withanolides of Ciplukan (Physalis angulata) In vitro
Retno Mastuti, Aminatun Munawarti, Mufidah Afiyanti
Asian Journal of Advances in Agricultural Research · pp. 7–17 · Published 12 Dec 2022
10.9734/ajaar/2022/v20i2392Abstract
This study aimed to determine the effect of in vitro polyploid induction using colchicine on the content of withanolides in Physalis angulata shoots. Polyploidy induction used two types of tissue, namely seeds from in vivo ripe fruit and in vitro cotyledonary nodes. Each tissue type was soaked in 0.1% colchicine for 0, 24, 48 and 72 hours. Seeds that had been soaked in colchicine were germinated in vitro and the cotyledonary nodes of seedling were used as explants for shoot induction. Meanwhile, the cotyledonary nodes that had been soaked in colchicine were immediately cultured on shoot induction medium. Media for shoot induction was MS + BAP 2 mg/L + IAA 0.05 mg/L. The shoots derived from multiplication were used for the analysis of withanolides content using the HPLC method. The results showed that a total of 38 withanolides compounds were detected in the retention time 21.6 to 36.8 minutes. Soaked the seeds in colchicine solution was more effective in increasing the withanolides content than the cotyledonary node tissues. All exposure time in colchicine only affected the intensity but had no effect on the profile of the withanolides.
References (34)
- 1 Some Results on Mean Square Error for Factor Score Prediction [DOI]
- 2 The polyploidy and its key role in plant breeding [DOI]
- 3 Polyploidy Affects Plant Growth and Alters Cell Wall Composition [DOI]
- 4 Studies on Colchicine Induced Chromosome Doubling for Enhancement of Quality Traits in Ornamental Plants [DOI]
- 5 In vitro Ploidy Manipulation for Crop Improvement [DOI]
- 6 Artificial polyploidy induction for improvement of ornamental and medicinal plants [DOI]
- 7 Effect of Polyploidy Induction on Natural Metabolite Production in Medicinal Plants [DOI]
- 8 Withanolides from the genus Physalis: a review on their phytochemical and pharmacological aspects [DOI]
- 9 Induced autopolyploidy—a promising approach for enhanced biosynthesis of plant secondary metabolites: an insight [DOI]
- 10 Genomic and ploidy manipulation for enhanced production of phyto-pharmaceuticals [DOI]
- 11 Chromosome doubling in a Rosa rugosa Thunb. hybrid by exposure of in vitro nodes to oryzalin: the effects of node length, oryzalin concentration and exposure time [DOI]
- 12 Cytotoxic Withanolide Constituents of Physalis longifolia [DOI]
- 13 Effects of ploidy level on the cellular, photochemical and photosynthetic characteristics in Lilium FO hybrids [DOI]
- 14 Physalins V-IX, 16,24-cyclo-13,14-seco withanolides from Physalis angulata and their antiproliferative and anti-inflammatory activities [DOI]
- 15 Effect of colchicine induced tetraploidy on morphology, cytology, essential oil composition, gene expression and antioxidant activity of Citrus limon (L.) Osbeck [DOI]
- 16 In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment [DOI]
- 17 Morphological Characteristics and Their Inheritance in Colchicine-induced Salvia Polyploids [DOI]
- 18 Cytotoxic Withanolides from the Whole Herb of Physalis angulata L. [DOI]
- 19 In vitro induction and generation of tetraploid plants of Sophora tonkinensis Gapnep [DOI]
- 20 Induction and Characterization of Tetraploids from Seeds ofBletilla striata(Thunb.) Reichb.f. [DOI]
- 21 Therapeutic Activities and Phytochemistry of Physalis Species Based on Traditional and Modern Medicine [DOI]
- 22 Effect of colchicine induced tetraploids of ginger (Zingiber officinale Roscoe) on cytology, rhizome morphology, and essential oil content [DOI]
- 23 In vitro induction of tetraploid plants from callus cultures of diploid bananas (Musa acuminata, AA group) ‘Kluai Leb Mu Nang’ and ‘Kluai Sa’ [DOI]
- 24 Production of tetraploid plants of Trollius chinensis Bunge induced by colchicine [DOI]
- 25 Treatment of Licorice Seeds with Colchicine: Changes in Seedling DNA Levels and Anthocyanin and Glycyrrhizic Acid Contents of Derived Callus Cultures [DOI]
- 26 Analysis of Major Withanolides inPhysalis longifoliaNutt. by HPLC-PDA [DOI]
- 27 Trifluralin-mediated polyploidization of fenugreek (Trigonella feonum-graecum L.) using in vitro embryo culture [DOI]
- 28 Identification of hybrids between triticale and Aegilops juvenalis (Thell.) Eig and determination of genetic similarity with ISSRs [DOI]
- 29 Phenotypic and molecular characterization of polyploidy Vicia faba induced by colchicine [DOI]
- 30 In Vitro Induction and Characterization of Polyploid Hydrangea macrophylla and H. serrata [DOI]
- 31 Studies on polyploidy induction for improvement of quality traits in ornamental and medicinal plants [DOI]
- 32 Polyploidy induction in Physalis alkekengi [DOI]
- 33 Medicinal Functions of Physalis Fruits for Biomedical Applications [DOI]
- 34 Austin Journal of Pharmacology and Therapeutics [DOI]
Cited by 0
No indexed citations yet.
Related research
- In vitro Impact Assessment of Aqueous Extract of Sida cordifolia Linn. Upon Rat Spermatozoa Parameters — shares topic coverage
- In vitro Probiotic Potential of Autochthonous Lactic Acid Bacteria and Microbiology of Kunu Made from Mixed Grains — shares topic coverage
- In vitro Investigation of Antimicrobial, Antitumor and DPPH Reduction Capacity of the Methanolic Extract of Scoparia dulcis — shares topic coverage
- Antifungal Efficacy of Zinc Oxide Nanoparticles against Anthracnose and Southern Blight Disease in Green Gram — shares topic coverage
- Evaluation of Fungicides against Sheath Rot Disease of Rice: An In vitro Study — shares topic coverage
Article metrics
Real usage data collected on this platform.
36
Page views
0
PDF downloads
5
Outbound clicks
0
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.