The Cytotoxic Effect of Small and Large Molecules of PMF Fraction Extracted from Camel Urine on Cancer Cells
Faiza A. Mahboub, Faten A. Khorshid, Abdul-Hamid M. Emwas
Journal of Advances in Medicine and Medical Research · pp. 384–396 · Published 16 Dec 2014
10.9734/BJMMR/2015/12271Abstract
Aim of the work: Animal urine, including that of camels, has long been used for the therapeutic management of human ailments. In this study, we sought to characterize the cytotoxic properties of newly derived purified fractions from previously described camel urine extract (PMF) on various cancer cell lines. Methodology: Two new size dissimilar fractions of PMF (large and small) were obtained by fractionalizing PMF using 3kD and 50kD membrane filters. A SRB cytotoxicity assay of the PMF fractions was performed on cancer cell lines (A549, HCT116, HepG2, MCF-7, U251 and Hela) as well as normal cell lines (human fibroblast cell line and Vero). Results: This study showed that the newly derived and more purified fraction of PMF (new PMF) possesses effective and selective anti-cancer properties against several types of cancer cell lines. Conclusion: This study, as well as previous ones, suggests that camel urine extracts (old and new PMF) may provide newer therapeutic alternatives to clinically manage cancer patients. However, further studies are needed to verify these positive preliminary results.
References (39)
- 1 Increase in signal-to-noise ratio of > 10,000 times in liquid-state NMR [DOI]
- 2 Water Suppression That Works. Excitation Sculpting Using Arbitrary Wave-Forms and Pulsed-Field Gradients [DOI]
- 3 Cisplatin as an Anti-Tumor Drug: Cellular Mechanisms of Activity, Drug Resistance and Induced Side Effects [DOI]
- 4 Detecting tumor response to treatment using hyperpolarized 13C magnetic resonance imaging and spectroscopy [DOI]
- 5 High-volume natural volcanic pozzolan and limestone powder as partial replacements for portland cement in self-compacting and sustainable concrete [DOI]
- 6 NMR-based metabolomics in human disease diagnosis: applications, limitations, and recommendations [DOI]
- 7 Comprehensive Extraction Method Integrated with NMR Metabolomics: A New Bioactivity Screening Method for Plants, Adenosine A1 Receptor Binding Compounds in Orthosiphon stamineus Benth [DOI]
- 8 Resolution Improvements inin Vivo1H NMR Spectra with Increased Magnetic Field Strength [DOI]
- 9 The application of micro-coil NMR probe technology to metabolomics of urine and serum [DOI]
- 10 Comparison of Artemisia annua Bioactivities between Traditional Medicine and Chemical Extracts [DOI]
- 11 Synthesis, spectral and structural characterization of dinuclear rhodium (II) complexes of the anticonvulsant drug valproate with theophylline and caffeine [DOI]
- 12 Real-Time High-Resolution X-ray Imaging and Nuclear Magnetic Resonance Study of the Hydration of Pure and Na-Doped C3A in the Presence of Sulfates [DOI]
- 13 Application of ex situ dynamic nuclear polarization in studying small molecules [DOI]
- 14 Determinants for Optimal Enhancement in Ex Situ DNP Experiments [DOI]
- 15 Solid-state 27Al nuclear magnetic resonance investigation of three aluminum-centered dyes [DOI]
- 16 Nuclear Magnetic Resonance Study of Nanoscale Ionic Materials [DOI]
- 17 Ethyl Acrylate‐Hydroxyethyl Acrylate and Hydroxyethyl Acrylate‐Methacrylic Acid: Reactivity Ratio Estimation from Cross‐linked Polymer Using High Resolution Magic Angle Spinning Spectroscopy [DOI]
- 18 Crystallization analysis fractionation of poly(ethylene-co-styrene) produced by metallocene catalysts [DOI]
- 19 Pharmacokinetics and Pharmacodynamics of Conjugated Equine Estrogens: Chemistry and Metabolism [DOI]
- 20 Complex Formation of the Antiviral 9‐[2‐(Phosphonomethoxy)Ethyl]Adenine (PMEA) and of Its N 1, N 3, and N 7 Deaza Derivatives with Copper(II) in Aqueous Solution [DOI]
- 21 Solid-state NMR paramagnetic relaxation enhancement immersion depth studies in phospholipid bilayers [DOI]
- 22 Solid state NMR and bioequivalence comparison of the pharmacokinetic parameters of two formulations of clindamycin [DOI]
- 23 Silica‐supported ( nBuCp ) 2ZrCl2 : effect of catalyst active center distribution on ethylene–1‐hexene copolymerization [DOI]
- 24 FT-IR spectroscopy as a tool for identification of apoptosis-induced structural changes in A549 cells treated with PM 701 [DOI]
- 25 Estrogen Deficiency is a Potential Cause for Osteopenia in Adult Male Patients with Noonan's Syndrome [DOI]
- 26 Apoptosis Study on the Effect of PMF on Different Cancer Cells [DOI]
- 27 Application to Classification of Mulberry Leaves using Multivariate Analysis of Proton NMR Metabolomic Data [DOI]
- 28 Dose Escalation Phase I Study in Healthy Volunteers to Evaluate the Safety of a Natural Product PM701 [DOI]
- 29 The Cytotoxic Effect of PM 701 and its Fractions on Cell Proliferation of Breast Cancer Cells, MCF7 [DOI]
- 30 Preclinical Evaluation of PM 701 in Experimental Animals [DOI]
- 31 An Ideal Selective Anti-Cancer Agent In Vitro: I-Tissue Culture Study of Human Lung Cancer Cells A549 [DOI]
- 32 Potential Anticancer Natural Product Against Human Lung Cancer Cells [DOI]
- 33 Hormone replacement therapy and urinary prostaglandins in postmenopausal women [DOI]
- 34 A General Model for the Optimization of Sample Processing Conditions by Solid-Phase Extraction Applied to the Isolation of Estrogens from Urine [DOI]
- 35 Biologic Effects of Delta-8-Estrone Sulfate in Postmenopausal Women [DOI]
- 36 Spectral analysis, molecular orbital calculations and antimicrobial activity of PMF-G fraction extracted from PM-701.
- 37 Applications of NMR-Based Metabonomic Approaches in the Assessment of Drug Toxicity
- 38 Pmf, Cesium And Rubidium Nanoparticles Induce Apoptosis In A549 Cells [DOI]
- 39 Cryogenic Probe 13C NMR Spectroscopy of Urine for Metabonomic Studies [DOI]
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