Effects of Methanolic Leaf Extracts of Daniella oliveri on Biochemical and Haematological Parameters of Albino Mice Infected with Plasmodium berghei NK 65

Abstract

The rapid emergence and spread of Plasmodium falciparum resistance to Artemisinin derivatives and all the conventional antimalarial drugs necessitates the importance of ethnobotany, resulting in need to study the antiplasmodial potentials and the resultant effects of the methanolic leaf extract of Daniella oliveri (D.oliveri) on the biochemical and haematological parameters of the infected and treated albino mice. A total of 30 mice were randomized to six groups; 1 (positive control), 2 (negative control), 3 (normal control), 4, 5 and 6 (treatment groups) of five mice per group, body weights of mice were measured before and after infection and treatments, the mice were Infected intravenously with 0.2 ml of 1x107 standard inoculum of chloroquine sensitive Plasmodium berghei infected erythrocytes on the first day (day 0), treatment commence 72 hours later (day 3), continued for 5 days to terminate on day 7. On day 8, the Swiss Albino mice used for antiplasmodial activity were subjected to euthanasia under chloroform, aseptically dissected and blood was collected through cardiac puncture in lithium heparin bottle for biochemical assays and in an ethylene diamine tetra- acetic acid (EDTA) bottles for haematological assays.All mice in the treatment group showed decrease in body weight except for normal control group that showed increase in body weight. Methanolic leaf extract of D.oliveri contains some secondary metabolites that are hepato-protective in nature with no significant effects on the biochemical and hematological parameters of the malaria infected and treated albino mice.