Synergistic Effect of Organic, Inorganic and Biofertilizers on Soil Microbial Activities in Rhizospheric Soil of Green Pea
Harleen Kaur, S. K. Gosal, S. S. Walia
Annual Research & Review in Biology · pp. 1–11 · Published 26 Apr 2017
10.9734/ARRB/2017/32509Abstract
To study the effect of incorporation of organic, inorganic and biofertilizers on rhizospheric microbial population and enzyme activities at various time intervals in pea crop, the experiment was laid in randomized blocked design in triplicate with plot size of 7 ×2.5 m2 using Punjab-88 variety. Study was conducted during winter season of 2015-16 in field of Department of Agronomy, Punjab Agricultural University, Ludhiana, India. Enumeration of bacteria, fungi, actinomycetes, diazotrophs, PGPR and PSB was done on Nutrient Agar medium, Glucose Yeast Extract medium, Kenknight’s medium, Jensen’s medium, King’s B medium and NBRIP medium respectively, using serial dilution spread plate technique. Activities of soil enzymes (Alkaline phosphatase, Urease and Dehydrogenase) were studied. Significantly higher total bacterial population (150 ×107 CFU g-1 of soil) and PGPR (218 ×105 CFU g-1 of soil) population was observed in treatments when there was combination of organic, inorganic and consortium biofertilizer. Actinomycetes remained unaffected with different fertilizers. Biofertilizer used in combination with FYM resulted in significantly higher population of fungi (20 ×103 CFU g-1 of soil), diazotrophs (140 ×105 CFU g-1 of soil) and PSB (80 ×104 CFU g-1 of soil). Among rhizospheric soil enzyme activities, alkaline phosphatase activity was significantly higher in treatment with FYM +consortium biofertilizer while dehydrogenase activity was higher in treatment with inorganic fertilizer +FYM +consortium biofertilizer. Urease activity showed a variable pattern during different time intervals. Microbial activities were also affected by different stages of plant growth. Total bacterial population showed positive correlation with enzyme activities during initial stages of plant growth but showed negative correlation with urease activity during late stages of plant growth. Combined application of organic and inorganic fertilizers resulted in improved soil microflora leading to significantly higher enzyme activities which were further enhanced by inoculation of biofertilizers.
References (18)
- 1 Use of p-nitrophenyl phosphate for assay of soil phosphatase activity [DOI]
- 2 Long-term effects of municipal solid waste compost application on soil enzyme activities and microbial biomass [DOI]
- 3 The effects of mineral fertilizer and organic manure on soil microbial community and diversity [DOI]
- 4 In search of biological indicators for soil health and disease suppression [DOI]
- 5 Influence of plant developmental stage on microbial community structure and activity in the rhizosphere of three field crops [DOI]
- 6 Responses of Bacterial Communities in Arable Soils in a Rice-Wheat Cropping System to Different Fertilizer Regimes and Sampling Times [DOI]
- 7 The potential of cropping systems and soil amendments for carbon sequestration in soils under long‐term experiments in subtropical India [DOI]
- 8 Microbial biomass and enzyme activities: Production and persistence [DOI]
- 9 Stability of urease in soils [DOI]
- 10 Relative changes in phosphatase activities as influenced by source and application rate of organic composts in field crops [DOI]
- 11 Biological effects of mineral nitrogen fertilization on soil microorganisms
- 12 Microbial community structure and nutrient availability in oil sands reclaimed boreal soils [DOI]
- 13 Yield, Soil Health and Nutrient Utilization of Field Pea (Pisum sativum L.) as Affected by Phosphorus and Bio-fertilizers under Subtropical Conditions of Jammu [DOI]
- 14 A rapid method of evaluating different compounds as inhibitors of urease activity in soils [DOI]
- 15 The influence of halophytic compost, farmyard manure and phosphobacteria on soil microflora and enzyme activities [DOI]
- 16 Effect of Biofertilzers on Growth and Yield Attributes of Pea (Pisum sativum L.)
- 17 Protective effect of Klebsiella bacteria on lawn grasses under conditions of soil salinization [DOI]
- 18 Soil Enzymes [DOI]
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