Supercritical CO2 Extraction and HPLC Quantification of Carnosic Acid from the leaves of Rosemary Officinalis: A Green Approach to Bioactive Compound Purification
Hemanth Kumar Manikyam, Dhanaseelan J, Jayaraj Mani
Chemical Science International Journal · pp. 16–25 · Published 9 Oct 2024
10.9734/CSJI/2024/v33i6920Abstract
This study explores the extraction and purification of carnosic acid, a potent antioxidant found in rosemary (Rosmarinus officinalis), using a green and sustainable method involving supercritical CO₂. The extraction was conducted at 45°C and 350 bar, with three cycles lasting 45 minutes each. The process resulted in the collection of 75 grams of oleoresin from 1 kg of dried rosemary leaves which is an optimal yield. The oleoresin underwent further purification via hexane precipitation, yielding a carnosic acid powder with a purity of 40% as determined by Gradient elution of Mobile phase A: Methanol: Water + Phosphoric acid solution, Mobile phase B: Methanol: Acetonitrile + Phosphoric acid solution by high-performance liquid chromatography (HPLC). Comparisons with a 60% purity standard highlight the potential of supercritical CO₂ extraction in obtaining bioactive compounds, though additional optimization is necessary to achieve higher purity.
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