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Research Article Open access CC BY 4.0

Sugar Cane Vinasse as a Renewable and Sustainable Feedstock for Lipid Production from Aspergillus Niger and Aspergillus flavus

Renata Pinto Ferreira Zaguini, Ana Bárbara Moulin Cansian, Jane Karla de Faria Borges Machado, Caroline Peters Pigatto De Nardi

Asian Journal of Biotechnology and Bioresource Technology · pp. 18–33 · Published 18 Jan 2024

10.9734/ajb2t/2024/v10i1195

Abstract

Background and Aims: In the search for sustainability and the use of renewable energies, biodiesel has stood out as a notable alternative to the growing energy demand of modern society. Microbial fat synthesis for lipids has been a possibility to produce this biofuel. In this context, the research work aimed to cultivate Aspergillus niger and Aspergillus flavus fungi in commercial and homemade broth, plus variable concentrations of sugarcane vinasse as a substrate to extract lipids produced using different extraction techniques and solvents. Methodology: The fungi were grown in a commercial medium and a homemade one made with potato extract. After cultivation, they were submitted to two pre-treatments: the Bead Mill technique and the Ultrasound technique. The extraction of lipids produced in a commercial medium was performed via Soxhlet with a mixture of solvents. Therefore, the lipids produced in a homemade medium were extracted with the adapted Bligh-Dyer method. Results: The species grew to the different culture media submitted, but the home culture medium increased the reproductive. The industrial residue proves to be a good alternative as a supplement to the culture medium, helping to reduce costs and preserve the environment. The extraction by Soxhlet showed satisfactory results when the fungi were grown in a commercial medium, with Aspergillus niger being extracted from the ethyl acetate solvent, obtaining a yield of 18.78%, and for A. flavus extracted with dichloromethane/methanol (9:1) and chloroform, bringing yield of 32.98% and 32%, respectively. Although extraction by Soxhlet showed some excellent results, extraction with the Bligh-Dyer method demonstrated better lipid fractions ranging from 25% to 67% and reduced energy expenditure, indicating a promising use, cost, and benefit. Conclusion: Filamentous fungi of the genus Aspergillus can be used to produce biodiesel as they proliferate and can produce the same amount of lipids as a vegetable, which requires a much larger area of soil, for example.

Aspergillus genus vinasse lipids fatty acids

References (22)

  1. 1 A comprehensive review on biodiesel as an alternative energy resource and its characteristics [DOI]
  2. 2 Sugarcane vinasse: Environmental implications of its use [DOI]
  3. 3 Biodiesel production from isolated oleaginous fungi Aspergillus sp. using corncob waste liquor as a substrate [DOI]
  4. 4 Microbial lipid production from potato processing wastewater using oleaginous filamentous fungi Aspergillus oryzae [DOI]
  5. 5 Seasonal variation of the organic and inorganic composition of sugarcane vinasse: main implications for its environmental uses [DOI]
  6. 6 Strategies to control pH in the dark fermentation of sugarcane vinasse: Impacts on sulfate reduction, biohydrogen production and metabolite distribution [DOI]
  7. 7 Biomethane Production from Sugarcane Vinasse in a Circular Economy: Developments and Innovations [DOI]
  8. 8 Solving the bottlenecks of sugarcane vinasse biodigestion: Impacts of temperature and substrate exchange on sulfate removal during dark fermentation [DOI]
  9. 9 Principles of Biochemistry. [DOI]
  10. 10 Lipid accumulation for biodiesel production by oleaginous fungus Aspergillus awamori: Influence of critical factors [DOI]
  11. 11 Does sugarcane vinasse composition variability affect the bioenergy yield in anaerobic systems? A dual kinetic-energetic assessment [DOI]
  12. 12 Toxicity of two effluents from agricultural activity: Comparing the genotoxicity of sugar cane and orange vinasse [DOI]
  13. 13 Treatment of sugarcane vinasse using an autochthonous fungus from the northwest of Argentina and its potential application in fertigation practices [DOI]
  14. 14 Latest advances and status analysis of nanomaterials for microalgae photosystem, lipids and biodiesel: A state of art [DOI]
  15. 15 Relating biomass composition and the distribution of metabolic functions in the co-fermentation of sugarcane vinasse and glycerol [DOI]
  16. 16 Bio-waste Transesterification Alternative for Biodiesel Production: A Combined Manipulation of Lipase Enzyme Action and Lignocellulosic Fermented Ethanol [DOI]
  17. 17 Evaluating granulometry and metal content in sludge from UASB reactors treating sugarcane vinasse [DOI]
  18. 18 Optimizing Laccase Production from Delonix regia Pods by Aspergillus carbonarius F5 Using Response Surface Methodology and it’s Dye Decolorization Potential [DOI]
  19. 19 Influence of Borassus flabellifer Endocarps Hydrolysate on Fungal Biomass and Fatty Acids Production by the Marine Fungus Aspergillus sp. [DOI]
  20. 20 A simple and promising route for biodiesel production from low-quality lipids [DOI]
  21. 21 Malic acid production by Aspergillus oryzae: the immobilized fungal fermentation route [DOI]
  22. 22 Investigating the Usefulness of Wild Dioscorea esculenta (Wild Yam) Flour as Substrate for Citric Acid Biosynthesis [DOI]

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