Skip to content
Research Article Open access CC BY 4.0

Study of Effect of Nutritional Factors for Optimization of Lipase Production by Lipolytic Fungi

Pratima Gupta, Nemashri Gupta, Vibhuti Rai, Piyush Parkhey

Microbiology Research Journal International · pp. 273–284 · Published 23 Oct 2014

10.9734/BMRJ/2015/5706

Abstract

Aims: To isolate lipase producing fungi from soil samples and study the effect of nutritional factors on lipase production by the isolated fungi. Study Design: Lipase producing fungi were isolated from oil contaminated soil samples. Different carbon, nitrogen and lipid sources and their different concentrations were tested for optimum lipase production. T-test and ANOVA were performed to validate the results. Place and Duration of the Study: Department of Biotechnology, National Institute of Technology, Raipur, From February to August, 2012. Methodology: Isolation of lipase producing fungi from oil contaminated soils was done using Saboraud’s agar medium. Identification of isolated fungi was done by Division of Plant Pathology, Indian Agriculture Research Institute, New Delhi. Different sources and concentrations of carbon, nitrogen and lipids (inducers) were tested for optimization of extracellular lipase production. Lipase assay was performed by photometric method. Tukey’s test and one way ANOVA tests were performed to validate the results obtained. Results: Three fungi isolated in the study were Aspergillus ochraceous, Aspergillus fumigatus and Penicillium purpurogenum. Culture amendments study indicated that maximum lipase activity for   A. ochraceous was reported with 0.5% maltose as carbon source and 0.5% peptone as nitrogen source. For A. fumigatus sucrose at 1.0% was the best carbon source, while soybean meal at 1.5% as nitrogen source reported maximum lipase activity. For P. purpurogenum maximum lipase activity was reported with 1.5% sucrose as carbon source and 1.5% peptone as nitrogen source. Tween 80 at 1.0% was the best inducer for all the three isolates. Conclusion: Lipase producing fungi were isolated from oil contaminated soils and culture amendments study was done to optimize extracellular lipase production.

Lipase nutritional modification ANOVA optimization

References (36)

  1. 1 PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT [DOI]
  2. 2 Biodiesel Fuel Production by Transesterification of Oils. [DOI]
  3. 3 Industrial applications of microbial lipases [DOI]
  4. 4 Production, purification, characterization, and applications of lipases [DOI]
  5. 5 Enzymatic biodiesel synthesis – Key factors affecting efficiency of the process [DOI]
  6. 6 Biocatalysis: Towards ever greener biodiesel production [DOI]
  7. 7 Purification strategies for microbial lipases [DOI]
  8. 8 Microbial Lipases and Their Industrial Applications: Review [DOI]
  9. 9 A novel alkaline lipase from Burkholderia cepacia for detergent formulation [DOI]
  10. 10 Extracellular enzymatic activity profiles in fungi isolated from oil-rich environments [DOI]
  11. 11 Effect of Nitrogen and Carbon Sources on Lipase Production by Penicillium aurantiogriseum
  12. 12 Improving lipase production by nutrient source modification using Rhizopus homothallicus cultured in solid state fermentation [DOI]
  13. 13 Industrial derivative of tallow: a promising renewable substrate for microbial lipid, single-cell protein and lipase production by Yarrowia lipolytica [DOI]
  14. 14 Effect of culture conditions on lipase production by Fusarium solani in batch fermentation [DOI]
  15. 15 Lipase Secretion and Citric Acid Production in Yarrowia lipolytica Yeast Grown on Animal and Vegetable Fat
  16. 16 Lipase Production by Penicillium restrictum in a Bench-Scale Fermenter [DOI]
  17. 17 Lipases from the genus Penicillium: Production, purification, characterization and applications [DOI]
  18. 18 Influence of cultivation conditions on the production of a thermostable extracellular lipase from Amycolatopsis mediterranei DSM 43304 [DOI]
  19. 19 Colorimetric determination of free fatty acids in biological fluids [DOI]
  20. 20 Production of Extracellular Lipases byPenicillium cyclopiumPurification and Characterization of a Partial Acylglycerol Lipase [DOI]
  21. 21 Production of lipase by soil fungi and partial characterization of lipase from a selected strain(Penicillium wortmanii) [DOI]
  22. 22 Optimization of lipase production by Penicillium simplicissimum in soybean meal [DOI]
  23. 23 Inoculum strategies for Penicillium simplicissimum lipase production by solid‐state fermentation using a residue from the babassu oil industry [DOI]
  24. 24 Production and partial characterisation of extracellular lipase from Aspergillus niger [DOI]
  25. 25 Lipase activity in Streptomycetes [DOI]
  26. 26 A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate [DOI]
  27. 27 Improving lipase production from Candida rugosa by a biochemical engineering approach [DOI]
  28. 28 Effect of various carbon sources on microbial lipases biosynthesis [DOI]
  29. 29 The effect of cultural conditions on the production of lipase by Acremonium strictum [DOI]
  30. 30 Lipase production byAspergillus andPenicillium species [DOI]
  31. 31 Enzymatic activities of Aspergillus fumigatus strains isolated from the air at waste landfills.
  32. 32 SCREENING AND IDENTIFICATION OF A NOVEL ALKALINE LIPASE PRODUCING BACTERIUM
  33. 33 Thermophilic Fungi from Nigerian Palm Produce [DOI]
  34. 34 Optimal design for the maximization of Penicillium cyclopium lipase production [DOI]
  35. 35 Production of Lipase by Certain Soil Fungi. I: Optimization of Cultural Conditions and Genetic Characterization of Lipolytic Strains of Aspergilli Using Protein Patterns and Random Amplified Polymorphic DNA (RAPD) [DOI]
  36. 36 Production of Extracellular Lipase from Aspergillus niger by Solid-State Fermentation

Cited by 6

Biotechnological valorization of oils from agro-industrial wastes to produce lipase using Aspergillus sp. from Amazon

M. V. Tacin, F. P. Massi, M. H. Fungaro · Biocatalysis and Agricultural Biotechnology · 2019

Showing 3 of 6 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

116

Page views

0

PDF downloads

64

Outbound clicks

6

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.