Structural Elucidation and Antimicrobial Analysis of Bioactive Compounds from Amaranthus spinosus
Anyanele, Sylvalyn Ijeoma, Ajiwe, Vincent Ishmael Egbulefu, Anyanele, Wisdom Chibuzo, Umeokoli, Blessing Ogechukwu
Asian Journal of Applied Chemistry Research · pp. 31–39 · Published 31 May 2023
10.9734/ajacr/2023/v13i4252Abstract
Amaranthus spinosus is a herb which belongs to the Amaranthaceae family and used by ethnic people in the treatment of various infections and ailments. As a result of its medicinal properties, it is gaining the attention of many researchers. This research work was carried out to isolate the compounds present in the leaves of Amaranthus spinosus, characterize, use obtained data to validate its bioactivity. The crude extract was obtained using methanol in a Soxhlet extractor. The pure extracts were isolated from the crude extracts using column chromatography and further purification was done using Sephadex. The structures of the isolates were elucidated using nuclear magnetic Resonance Spectroscopy. In the validation of the bioactivity of the plant, some pathogens were used, including Escherichia coli, Bacillus species and Pseudomonas aeruginosa, Candida albicans and Aspergillus niger. The antimicrobial analysis carried out using two isolates from the plant extracts indicated the following zones of inhibition (mm) for the first isolate: 16.00 ± 0.01, 16.00 ±0.01, 15.00 ± 0.08, 16.00±0.08, 17.00 ± 0.08. For the second isolate, the diameter zone of inhibition (mm) includes 6.00±0.08, 5.00± 0.10, 26.00± 0.10, 5.00±0.08, and 16.00±0.10. These results were compared with a standard antibiotic, ciprofloxacin, and standard antifungal, ketoconazole. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) of the isolated compounds were determined and compared with those of the standard drugs.
References (11)
- 1 Phytochemical constituents of some Nigerian medicinal plants [DOI]
- 2 Well diffusion for antifungal susceptibility testing [DOI]
- 3 General overview of medicinal plants: A review [DOI]
- 4 Indigenous knowledge and uses of medicinal plants by local communities of the Kali Gandaki Watershed Area, Nepal [DOI]
- 5 Ethnobotanical survey for potential anti-malarial plants in south-western Nigeria [DOI]
- 6 Evaluation of gut modulatory and bronchodilator activities of Amaranthus spinosus Linn. [DOI]
- 7 Antibacterial, Cytotoxic and Antioxidant Activity of Chloroform, n-hexane and Ethyl Acetate extract of plant Amaranthus spinosus
- 8 Antidiarrheal and antiulcer activity ofAmaranthus spinosusin experimental animals [DOI]
- 9 Documentation and consensus of indigenous knowledge on medicinal plants used by the local communities of western Uganda
- 10 Prevention of type 2 diabetes in the prediabetic population.
- 11 Indian Medicinal Plants [DOI]
Cited by 8
Anyanele, Sylvalyn Ijeoma, Ajiwe, Vincent Ishmael Egbulefu, Anyanele, Wisdom Chibuzo, Umeokoli, Blessing Ogechukwu, Modozie, Benjamin Uchenna · Asian Journal of Chemical Sciences · 2023
Igor L. Bretas, Jose C. B. Dubeux, Luana M. D. Queiroz · Soil Science Society of America Journal · 2025
Sumathy Rengarajan, Renuga Devi Navaneethan, S. Alagendran · Luminescence · 2025
Carlos Barba-Ostria, Jéssica Guamán-Bautista, Augusto A. Tosi-Vélez · Nutrients · 2025
Showing 4 of 8 known citations — external sources report more than can currently be individually listed.
Related research
- Antimicrobial Assessment of Annona muricata Fruits and Its Chemical Compositions — shares topic coverage
- Knowledge and Perception of HealthCare Workers on Antibiotic Resistance and Predictors of Antibiotic Prescription in Benue State, Nigeria — shares topic coverage
- Preliminary Phytochemical Screening and Physiochemical Studies of Eucalyptus camaldulensis and Ocimum tenuiflorum — shares topic coverage
- Antibacterial and Phytochemical Activity of Gliricidia sepium against Poultry Pathogens — shares topic coverage
- Incidence of Enterobacteriaceae in Retail Meat Samples and Their Antibiotic Susceptibility — shares topic coverage
Article metrics
Real usage data collected on this platform.
14
Page views
0
PDF downloads
1
Outbound clicks
8
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.