Statistical Optimization of Culture Media and Conditions for Maximize Production of Mannan by Saccharomyces Cerevisiae Using Response Surface Methodology
Vakilian Aghooi Hanneh, Mortazavi Seyed Ali, Milani Elnaz, Koocheki Arash, Mehraban Masoome
Annual Research & Review in Biology · pp. 1927–1940 · Published 3 Mar 2014
10.9734/ARRB/2014/8712Abstract
Aim: In view of the increase in Saccharomyces cerevisiae mannan content, the culture medium and condition for S. cerevisiae were optimized in this study. Study Design: The mathematical model was established by the quadratic rotary combination design via Response Surface Methodology to evaluate the effect of culture condition and media on mannan content in the yeast cell wall. Methodology: The influence of culture medium ingredients such as carbon source (FOS, in the range of 2 – 9 g/100ml) and nitrogen source (mixture of peptone and yeast extract, in the range of 1-5g/100ml), and enzyme activator (glycerol, in the range of 1-4g/100ml) on mannan production were evaluated using Response surface methodology. Also The influence of original pH (in the range of 4-7), inoculums size (in the range of 2-4%) and temperature (in the range of 26-32ºC), on mannan production was evaluated and confirmed by quadratic design. Results: The optimized concentrations of culture medium were determined as follows: 8.99 g/100mL, FOS; 4.25 g/100 ml, mixture of bacto peptone and yeast extract; and 3 g/100mL, glycerol. The order of effect was carbon source› nitrogen source› enzyme activator. Also The order of effect of original pH, inoculums size, and temperature, on mannan production was as follows: temperature > initial pH > inoculation size. The optimized culture condition was pH, 6.62; inoculums size, 4 ml; temperature, 32ºC. The maximum mannan production increased to 94.912 ±9.1 mg/100 ml at the optimum culture condition. Conclusion: It was evident that the mannan production was affected significantly by culture medium and condition optimization (p < 0.01).
References (19)
- 1 Biostatistics: A Foundation for Analysis in the Health Sciences. [DOI]
- 2 Agricultural Experimentation: Design and Analysis [DOI]
- 3 Review: Cell wall assembly in yeast [DOI]
- 4 A study of the yeast cell wall composition and structure in response to growth conditions and mode of cultivation [DOI]
- 5 A new method for quantitative determination of polysaccharides in the yeast cell wall. Application to the cell wall defective mutants ofSaccharomyces cerevisiae [DOI]
- 6 Optimization of medium and cultivation conditions for pullulan production by a new pullulan-producing yeast strain [DOI]
- 7 Introduction to Probability and Statistics [DOI]
- 8 Optimization of agitation, aeration, and temperature conditions for maximum β-mannanase production [DOI]
- 9 Optimization of medium for the production of β-cyclodextrin glucanotransferase using Central Composite Design (CCD) [DOI]
- 10 Structure of the Linkage Region between the Polysaccharide and Protein Parts of Saccharomyces cerevisiae Mannan [DOI]
- 11 Chemical and structural alterations at the cell surface of Candida tropicalis, induced by hydrocarbon substrate [DOI]
- 12 Carbon source-dependent transcriptional regulation of the mitochondrial glycerol-3-phosphate dehydrogenase gene,GUT2, fromSaccharomyces cerevisiae [DOI]
- 13 Immunoreactivity of the fungal cell wall [DOI]
- 14 Medium optimization of carbon and nitrogen sources for the production of eucalyptene A and xyloketal A from Xylaria sp. 2508 using response surface methodology [DOI]
- 15 Statistical optimization of culture media and conditions for production of mannan by Saccharomyces cerevisiae [DOI]
- 16 Chemical and enzymatic changes in the cell walls of Candida albicans and Saccharomyces cerevisiae by scanning electron microscopy [DOI]
- 17 Alterations in the cell wall ofSaccharomyces cerevisiae induced by the alpha sex factor or a mutation in the cell cycle [DOI]
- 18 Impact of magnesium and mannose in the cultivation media on the magnesium biosorption, the biomass yield and on the cell wall structure of Candida utilis yeast [DOI]
- 19 Effect of anticalmodulin drugs on the action of yeast ? factor pheromone [DOI]
Cited by 4
A. Hajhosseini, A. Sharifan, Zohre Eftekhari · Iranian Biomedical Journal · 2023
Shobha Gupta, Z. Bhathena · International Journal of Current Microbiology and Applied Sciences · 2020
Showing 2 of 4 known citations — external sources report more than can currently be individually listed.
Related research
- Microbial Synthesis of Polyhydric Alcohol by Saccharomyces cerevisiae — shares topic coverage
- Influence of Saccharomyces cerevisiae (Baker’s Yeast) on the Fermentation of Ogi-A Nigerian Fermented Food — shares topic coverage
- An Electron Microscopic Study of the Effect of (Saccharomyces cerevisiae) on the Ability of E. coli 0157:H7 to Attach and Efface Healthy Young Broilers — shares topic coverage
- Comparative Studies on Production of Bioethanol from Rice Straw Using Bacillus subtilis and Trichoderma viride as Hydrolyzing Agents — shares topic coverage
- Evaluation of C19 Steroid Intermediates during Microbial Transformation of Phytosterol — shares topic coverage
Article metrics
Real usage data collected on this platform.
1
Page views
0
PDF downloads
0
Outbound clicks
4
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.