Stability and Kinetics Studies Using an  RP- HPLC-UV Method Developed for Assays of Salvianolic Acid A Degradation as a Therapeutic

Were L. L. Munyendo; Lilechi D. Baraza; George Sowayi

doi:10.9734/IRJPAC/2015/15348

Research Article Open access CC BY 4.0

Stability and Kinetics Studies Using an RP- HPLC-UV Method Developed for Assays of Salvianolic Acid A Degradation as a Therapeutic

Were L. L. Munyendo, Lilechi D. Baraza, George Sowayi

International Research Journal of Pure and Applied Chemistry · pp. 99–109 · Published 13 Mar 2015

10.9734/IRJPAC/2015/15348

Abstract

Aims: To evaluate the stability of Salvianolic acid A (SAA), a promising cardiovascular drug candidate. Additionally avail an archetype in-vitro therapeutic monitoring from SAA degradation profile for characteristic oral gavages. Study Design: Experimental by laboratory analysis. Place and Duration of Study: Department of Chemistry & Biochemistry, Moi University; Department of Pure & Applied Chemistry and Department of Medical Laboratory Sciences of Masinde Muliro University of Science & Technology, between June 2013 and September 2014. Methodology: Drug stability was studied according to ICH guidelines. A stability-indicating HPLC method was developed and validated; mimicking the systemic environs SAA degradation kinetics was then evaluated. Results: SAA degradation followed first-order kinetics with rate constant increasing from 0.0099 h−1 at 313 K to 0.08044 h−1 at 363 K. The t0.5 was between 70.0 and 9.8 hours while t0.9 was between 10.6 and 1.5 hours within 313 K to 363K temperature range. Activation energy was 39.56 KJmol-1. A V-shaped pH-rate profile was observed with maximum stability at pH4.0. Degradation was rapid in hydrogen peroxide solution and in the simulated gastro-intestinal fluids. SAA exhibits high stability at pH 4.0, hence the suggested optimum pH for processing. Conclusion: This study provides sufficient physicochemical data depicting SAA to be of intermediate stability. Therefore availing an imperative basis for selecting suitable dosage forms and expected kinetics during therapeutic SAA monitoring.

Salvianolic acid A stability therapeutic degradation kinetics

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Were L. L. Munyendo, Lilechi D. Baraza, George Sowayi (2015). Stability and Kinetics Studies Using an RP- HPLC-UV Method Developed for Assays of Salvianolic Acid A Degradation as a Therapeutic. International Research Journal of Pure and Applied Chemistry , 99–109. https://doi.org/10.9734/IRJPAC/2015/15348

Affiliations

Were L. L. Munyendo — Department of Chemistry and Biochemistry, Moi University, P.O.Box 3900-30100, Eldoret, Kenya and Department of Medical Laboratory Sciences, Masinde Muliro University of Science and Technology, P.O.Box 190-50100, Kakamega, Kenya
Lilechi D. Baraza — Department of Pure and Applied Chemistry, Masinde Muliro University of Science and Technology, P.O.Box 190-50100, Kakamega, Kenya
George Sowayi — Department of Medical Laboratory Sciences, Masinde Muliro University of Science and Technology, P.O.Box 190-50100, Kakamega, Kenya

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