Development of In vitro Tetraploid Plants of  Hevea brasiliensis

U. K. Divya; S. Sushama Kumari

doi:10.9734/ijpss/2019/v28i630125

Research Article Open access CC BY 4.0

Development of In vitro Tetraploid Plants of Hevea brasiliensis

U. K. Divya, S. Sushama Kumari

International Journal of Plant & Soil Science · pp. 1–12 · Published 16 Jul 2019

10.9734/ijpss/2019/v28i630125

Abstract

Increase in global consumption of natural rubber necessitates crop improvement of Hevea aimed at increased productivity. As conventional breeding of Hevea is very elaborate and time consuming. Hence in the present study development of tetraploids through chromosome doubling of diploid callus obtained from cultured immature inflorescence of Hevea using colchicines were attempted. Chromosome doubling of the diploid callus occurred when treated with 1.25 µM colchicine for 3 days. In higher concentrations as well as at longer exposure periods, the callus texture and viability were affected. 48 % embryo induction and a maturation frequency of 45 % were obtained. Embryo germination and plant regeneration with a germination frequency (30 %) and a regeneration frequency (20 %) were obtained. Cytological and flow cytometric analyses confirmed the tetraploid nature of the colchicines treated callus. In vitro tetraploid plant developed through these in vitro techniques can be further used in Hevea brasiliensis breeding.

Hevea brailiensis colchicine in vitro tetraploids

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U. K. Divya, S. Sushama Kumari (2019). Development of In vitro Tetraploid Plants of Hevea brasiliensis. International Journal of Plant & Soil Science , 1–12. https://doi.org/10.9734/ijpss/2019/v28i630125

Affiliations

U. K. Divya — Indian Academy Degree College Autonomous, Bangalore, India and Rubber Research Institute of India-686 009, Kerala, India.
S. Sushama Kumari — Rubber Research Institute of India-686 009, Kerala, India.

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