Skip to content
Research Article Open access CC BY 3.0

Role of Different Additives on Survival of Serratia plymuthica HRO-C48 on Oilseed Rape Seeds and Control of Phoma lingam

Ruba Abuamsha, Mazen Salman, Ralf-Udo Ehlers

Microbiology Research Journal International · pp. 737–748 · Published 21 Mar 2014

10.9734/BMRJ/2014/8390

Abstract

For optimum inhibition of plant pathogens, biocontrol agents must be maintained at higher density and survive for a long time in the plant system. Biopriming of seeds with bacteria in the presence of different additives provide a promising technique that might improve the efficacy of biocontrol agents and their application. The aim of this work was to test different additives and stickers on the activity of Serratia plymuthica in oilseed rape in controlling Phoma lingam. Seeds were soaked in bacterial suspensions (log10 11 CFU ml-1) containing one of the following Stickers and additives in a ratio of 1:1:1 (w:v:v): Sodium alginate, Dextran T 40, Polyvinyl alcohol, Methylcellulose, Gum Arabic, Raffinose, Tween 20, and Paraffin oil. Seeds were stored either at room temperature or at 4°C. Number of bacteria inside the seeds was monitored over a period of 12 months. The effect of additives and stickers on bacterial efficacy in controlling Phoma lingam was evaluated in pot experiments. Number of bacterial cells inside the seeds was significantly higher in the seeds coated with GA+MgSO4, RF+MC and PA (log10 7.5 ± 0.2, log10 7.5 ± 0.2 and log10 7.4 ± 0.4, respectively). Interestingly, bacterial concentration in seeds stored at 4°C was higher than that in seeds stored at 20°C. Moreover, after storage for 8 months, Serratia plymuthica was able to control the black leg disease. Our results showed that some additives and stickers prolonged the shelf life of bacteria inside the seeds and improved the efficacy of bacteria in controlling the disease.

Biological control bacteria plant pathogens additives stickers

References (24)

  1. 1 Natural Plant Protection by 2,4-Diacetylphloroglucinol-Producing Pseudomonas spp. in Take-All Decline Soils [DOI]
  2. 2 MICROBIAL DYNAMICS AND INTERACTIONS IN THE SPERMOSPHERE [DOI]
  3. 3 Concepts And Technologies Of Selected Seed Treatments [DOI]
  4. 4 Dose-Response Relationships in Biological Control of Fusarium Wilt of Radish byPseudomonasspp. [DOI]
  5. 5 Paenibacillus polymyxaproduces fusaricidin-type antifungal antibiotics active againstLeptosphaeria maculans, the causative agent of blackleg disease of canola [DOI]
  6. 6 Biopriming of Infected Carrot Seed with an Antagonist, Clonostachys rosea, Selected for Control of Seedborne Alternaria spp. [DOI]
  7. 7 Impact of formulation procedures on the effect of the biocontrol agent Serratia plymuthica HRO-C48 on Verticillium wilt in oilseed rape [DOI]
  8. 8 SMP: Solid matrix priming of seeds [DOI]
  9. 9 Menadione sodium bisulphite: a novel plant defence activator which enhances local and systemic resistance to infection by Leptosphaeria maculans in oilseed rape [DOI]
  10. 10 Responses of non-primed or primed seeds of ‘Marketmore 76’ cucumber (Cucumis sativus L.) slurry coated with Trichoderma species to planting in growth media infested with Pythium aphanidermatum [DOI]
  11. 11 Effect of seed priming with Serratia plymuthica and Pseudomonas chlororaphis to control Leptosphaeria maculans in different oilseed rape cultivars [DOI]
  12. 12 Improvement of the formulation and antagonistic activity of Pseudomonas fluorescens B5 through selective additives in the pelleting process [DOI]
  13. 13 Population Dynamics of Biocontrol Agents and Pathogens in Soils and Rhizospheres [DOI]
  14. 14 Application of Beneficial Microorganisms to Seeds during Drum Priming [DOI]
  15. 15 Beneficial microorganism survival on seed, roots and in rhizosphere soil following application to seed during drum priming [DOI]
  16. 16 Effect of Seed Quality and Combination Fungicide-Trichoderma spp. Seed Treatments on Pre- and Postemergence Damping-Off in Cotton [DOI]
  17. 17 Comparison of application methods to prolong the survival of potential biocontrol bacteria on stored sugar-beet seed [DOI]
  18. 18 Seed Treatment Using Pre-infiltration and Biocontrol Agents to Reduce Damping-off of Corn Caused by Species of Pythium and Fusarium [DOI]
  19. 19 Bacterial Antagonist as Seed Treatment to Control Leaf Blight Disease of Bambara Groundnut (Vigna subterranea) [DOI]
  20. 20 Differential resistance of oilseed rape cultivars (Brassica napus ssp. oleifera) to Verticillium longisporum infection is affected by rhizosphere colonisation with antagonistic bacteria, Serratia plymuthica and Pseudomonas chlororaphis [DOI]
  21. 21 Efficacy of Stickers for Seed Treatment with Organic Matter or Microbial Agents for the Control of Damping-off of Sugar Beet
  22. 22 Antagonism of Erwinia herbicola towards Leptosphaeria maculans causing blackleg disease of Brassica napus [DOI]
  23. 23 Effectiveness of Bacterial and Fungal Isolates to Control Phoma lingam on Oilseed Rape Brassica napus [DOI]
  24. 24 Biological control of Botrytis cinerea on chickpea seed with Trichoderma spp. and Gliocladium roseum : indigenous versus non‐indigenous isolates [DOI]

Cited by 14

Emerging Roles of Biopolymers in Seed Science and Technology

Anu, Manonmani Velusamy, Sivakumar Rathinavelu · Biopolymers · 2025

Biopriming salt-tolerant microbial isolates to chilli and pak choy seeds: a study on salinity tolerance and physiological responses of treated seeds

J. Chin, A. Ting · Acta Biologica Szegediensis · 2023

Biopriming chilli seeds with Trichoderma asperellum: A study on biopolymer compatibility with seed and biocontrol agent for disease suppression

J. Chin, Y. Lim, A. Ting · Biological control (Print) · 2022

Biopriming Pseudomonas fluorescens to vegetable seeds with biopolymers to promote coating efficacy, seed germination and disease suppression

J. Chin, Y. Lim, A. Ting · Journal of the Saudi Society of Agricultural Sciences · 2022

Bacterial and fungal endophyte communities in healthy and diseased oilseed rape and their potential for biocontrol of Sclerotinia and Phoma disease

C. Schmidt, L. Mrnka, P. Lovecká · Scientific Reports · 2021

Biopolymers for biopriming of Brassica rapa seeds: A study on coating efficacy, bioagent viability and seed germination

J. Chin, Y. Lim, A. Ting · Journal of the Saudi Society of Agricultural Sciences · 2021

Biological control of potato soft rot caused by Dickeya solani and the survival of bacterial antagonists under cold storage conditions

I. Hadizadeh, B. Peivastegan, Asko Hannukkala · Plant Pathology · 2018

Showing 7 of 14 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

84

Page views

0

PDF downloads

56

Outbound clicks

14

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.