Antioxidant and Anti-arthritic Potential of Casuarina equisetifolia Fruit Methanolic Extract

Abstract

Aims: Antioxidant and anti-arthritic potential of Casuarina equisetifolia fruit methanolic extract. Place: C.U. Shah College of Pharmacy and Research, Wadhwan, Surendranagar, Gujarat, India. Methodology: Extract was subjected to qualitative and quantitative investigation then antioxidant properties of extract was determine by two method namely Nitric Oxide free radical scavenging activity and Reducing power by FeCl3 method. Based on Phytochemical and antioxidant result anti-arthritic activity was performed on wistar rats using Complete Freund’s Adjuvant (CFA) and evaluated different parameter like paw volume, arthritic index, biochemical parameter and hematological parameter. Results: In this study found that the methanolic extract of Casuarina equisetifolia fruit (MLCEF) contain significant percentage of secondary metabolite like poly phenol that properties was proved antioxidant activity. Antioxidant properties was determined by two methods the MLCEF IC50 Value 30.27±2.43 and 158.45±7.15 was found IC50 value reveled that MLCEF is a potent free radical scavenge capacity. Casuarina equisetifolia fruit (MLCEF) of 400 mg/kg, p.o. showed significant increasement in body wt from day 12th to 21st and significantly reduction in rat paw edema was observed from day 6th to 21st and it could be significantly normalize the haematological (like Hb, WBC and ESR) and biochemical abnormalities (like urea, ALT, AST and ALP) adjuvant induced arthritic rats in both developing and developed phases of CFA induced arthritis.          Conclusion: The study outcome reveled that MLCEF is contain significant amount of polyphenol and that properties exhibit good antioxidant properties and significant antiarthritic properties. The actual mechanism of action of extracts on adjuvant induced arthritis is not clear with these studies. The action of extracts on proinflammatory mediators like TNF-α, Interleukins and other relevant mediators will be carried out in future to study its mechanism.