Preclinical Immunogenicity of Capsule and Outermembrane Proteins of a Local Neurotropic Isolate of Hemophilus Infleunzae B in a Lapin Model

Abstract

The preclinical immunogenicity of capsule C and outer membrane protein OMP of human neurotropic isolate of Hemophilus influenzae b HIB in a lapin model is being reported. Hemophilus influenzae b is gram negative, short rods, encapsulated bacterium, microaerophilic, fastidious, need X and V factor for growth with six capsular serotypes. Among which serotype b was the dominant. Itis associated with human pyogenic respiratory and meningeal infections. The C and OMP were separated, purified, and characterized. Then, quantfied to the rate of 3 mg/m L and 2.27 mg/mL accordingly. Both of the preparations were used as test antigens for the invitro use and for speific immune priming of rabbits. Cell free culture filtrate of test isolate was prepared and used as a skin sensitin in DTH test. Rabbits were were preconditioned with CFA adjuvant for two weeks then specific immune primed using multisite multi-injection protocols. Capsule immunogen induce leukocyte stimulatory factor cytokines. Four to six folds rise both titres and concentrations of anti capsular precipitins and anti OMP hemagglutinins were noted. Both C and AMP are proved to be lapin immunogens stimulate humoral immune responses at both systemic and mucosal immune compartments. These findings may forms the background information and be an integral part of preclinical and clinical development and production of local H. infleunzae b systemic and/or mucosal vaccines. Which could be valid on approval for local mass vaccination against childhood meningitis in this area.