Relationship between Glutathione Levels and Activities of Detoxification Enzymes in Anopheles gambiae from Northwest Nigeria
Current Journal of Applied Science and Technology · pp. 1–11 · Published 9 Sep 2015
10.9734/BJAST/2015/19851Abstract
Aims: The aim of this study is to investigate the relationship between levels of the three forms of glutathione and the activities of detoxification enzymes in Anopheles gambiae from northwest Nigeria. Study Design: Anopheles gambiae larvae were sampled from breeding sites with marked differences in physico-chemical characteristics, grouped into three different study zones A, B & C on the bases of human related activities (intensive agriculture, domestic, and petrochemical activities, respectively) taking place within and/or around the breeding sites. The sampled larvae were reared until they emerged into pupae and adult. Place and Duration of Study: Department of Biochemistry Bayero University Kano, Abertay Centre for Environment, University of Abertay Dundee, between June 2011 and May 2013. Methodology: Activities of the 3 major detoxification enzymes (P450, GST and α & β-esterases) were assayed on the sampled larvae and the emerged pupae and adult followed by the determination of the levels of total, oxidized and reduced glutathione across the three life stages. Results: Following various statistical analyses, the activities of the detoxification enzymes were higher in zones A and C (which also recorded higher levels of the physico-chemical factors) compared to zone B. Also, the relative distribution of glutathione showed that the levels of total and reduced GSH across the three study zones appeared to be similar while the levels of oxidized glutathione (GSSG) appeared to be higher in study zone A and C compared to zone B. A deduced statistical model established the different forms of the glutathione variables as having a significant effect on the activities of the detoxification enzymes across the three life stages of An gambiae from northwest Nigeria. Conclusion: These observations could allow for an evaluation of the possibility of targeting control of the activities of detoxification enzymes in mosquitoes through manipulation of the availability of GSH in the management of insecticides resistance in mosquitoes.
References (11)
- 1 Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues [DOI]
- 2 The economic and social burden of malaria [DOI]
- 3 Africans. The History of a Continent [DOI]
- 4 Cross-induction of detoxification genes by environmental xenobiotics and insecticides in the mosquito Aedes aegypti: Impact on larval tolerance to chemical insecticides [DOI]
- 5 Benzo[a]pyrene-induced elevation of GSH level protects against oxidative stress and enhances xenobiotic detoxification in human HepG2 cells [DOI]
- 6 Determination of total and oxidized glutathione in human whole blood with a sequential injection analysis system [DOI]
- 7 Insulin Signaling Regulates γ-Glutamylcysteine Ligase Catalytic Subunit Expression in Primary Cultured Rat Hepatocytes [DOI]
- 8 Larval Productivity and Detoxification Enzymes Profile in Response to Physico-chemical Environmental Factors of Anopheles gambiae Breeding Ecologies in Nigeria [DOI]
- 9 Glutathione Levels during the Mosquito Life Span with Emphasis on Senescence [DOI]
- 10 Creatures of Our Own Making [DOI]
- 11 Capacity of glutathione in enzymatic detoxification of tetramethrin metabolites
Cited by 0
No indexed citations yet.
Related research
- Risk Factors Associated with Pneumonia among under 5 Years Children at Banadir Hospital, Mogadishu, Somalia — shares topic coverage
- Unraveling the Complex Dynamics of Soil Microbiome Diversity and Its Implications for Ecosystem Functioning: A Comprehensive Review — shares topic coverage
- Antibiotic Resistance: An Environmentally Regulated Threaten Crisis — shares topic coverage
- Epidemiological Factors Influencing the Development of Bacterial Blight in Clusterbean in Semi Arid Eastern Plain Zone –IIIA of Rajasthan, India — shares topic coverage
- Larval Productivity and Detoxification Enzymes Profile in Response to Physico-chemical Environmental Factors of Anopheles gambiae Breeding Ecologies in Nigeria — shares topic coverage
Article metrics
Real usage data collected on this platform.
85
Page views
0
PDF downloads
43
Outbound clicks
0
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.