Rapid Detection of Airborne Inocula of Grapevine Mildews Using PCR and LAMP Assay
J. Shajith Basha, A. Kamalakannan, Basha, J. Shajith, S. Saraswathy, I. Johnson, Patil Santosh Ganapati, K. R. Swarna Lakshmi
International Journal of Plant & Soil Science · pp. 12–21 · Published 27 Oct 2021
10.9734/ijpss/2021/v33i2230677Abstract
Grapes powdery mildew and downy mildew caused by Erysiphe necator and Plasmopara viticola respectively are the most devastating diseases worldwide resulting in significant loss of yield and quality. Epidemics of grapevine mildews are caused by airborne inocula such as conidia and sporangia. Rapid detection of airborne inocula will help to face up timely management strategies under field conditions. The aim of the current study was to design a suction spore trap to trap the airborne mildew inocula and their early detection by molecular methods of PCR and LAMP assay. A total of twelve airborne inocula samples were collected the weekly intervals from 3 to 14 standard weeks of 2021 during the cropping season. The presence of airborne inocula of E. necator was detected on standard weeks 3,6,10 and 13 through PCR assay which yielded an amplicon of 470 bp. Similarly, airborne inocula of P.viticola were detected on standard week 6 only through PCR which yielded an amplicon of 520 bp. A rapid, highly specific , sensitive Loop mediated isothermal amplification (LAMP) assay was performed to detect the E. necator and P. viticola using six sets of LAMP primers constructed by targeting rDNA region of ITS and the 5S rRNA and CesA4 a gene, respectively. LAMP assay efficiently detected the presence of airborne inocula of E.necator in most of the samples collected from standard week 3 – 14 except 7, 8, and 9. However, the presence of airborne inocula of P.viticola from standard week 3 – 14 was confirmed by LAMP assay. The LAMP assay is absolutely the best in identifying airborne inocula of grapevine mildews compared to PCR and phenotypic microscopic observation.
References (22)
- 1 Loop-mediated isothermal amplification of DNA [DOI]
- 2 Accelerated reaction by loop-mediated isothermal amplification using loop primers [DOI]
- 3 Detection of Loop-Mediated Isothermal Amplification Reaction by Turbidity Derived from Magnesium Pyrophosphate Formation [DOI]
- 4 Colorimetric Detection of Loop-Mediated Isothermal Amplification Reaction by Using Hydroxy Naphthol Blue [DOI]
- 5 Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases [DOI]
- 6 Innovations in air sampling to detect plant pathogens [DOI]
- 7 Detection of Botrytis cinerea by loop-mediated isothermal amplification [DOI]
- 8 Grapevine Powdery Mildew: Fungicides for Its Management and Advances in Molecular Detection of Markers Associated with Resistance [DOI]
- 9 Development of a grower‐conducted inoculum detection assay for management of grape powdery mildew [DOI]
- 10 Effects of Humidity on the Development of Grapevine Powdery Mildew [DOI]
- 11 Genetic Variation in Powdery Mildew of Barley: Development of RAPD, SCAR, and VNTR Markers [DOI]
- 12 An Optimized Protocol for DNA Extraction from Wheat Seeds and Loop-Mediated Isothermal Amplification (LAMP) to Detect Fusarium graminearum Contamination of Wheat Grain [DOI]
- 13 Development and application of loop-mediated isothermal amplification (LAMP) for detection of Plasmopara viticola [DOI]
- 14 Loop‐mediated isothermal amplification assay for the detection of Plasmopara viticola infecting grapes [DOI]
- 15 Spatiotemporal Relationships Between Disease Development and Airborne Inoculum in Unmanaged and Managed Botrytis Leaf Blight Epidemics [DOI]
- 16 Rapid detection of Colletotrichum gloeosporioides using a loop-mediated isothermal amplification assay [DOI]
- 17 Monitoring of ascospore density of Erysiphe necator in the air in relation to weather factors and powdery mildew development [DOI]
- 18 Advancing technologies in gene amplification [DOI]
- 19 Evaluation of a dynamic model for primary infections caused by Plasmopara viticola on grapevine in Quebec
- 20 International Journal of Molecular Sciences [DOI]
- 21 Biochemical and Biophysical Research Communications [DOI]
- 22 Journal of Infection and Chemotherapy [DOI]
Cited by 24
Abhay Kumar Pandey, Martin J. Barbetti, Kuldeep Tripathi · Plant Pathology · 2025
Sara Leoni, Odile Carisse, Livio Ruzzante · 2025
Ravindra Kumar, Seweta Srivastava, Aishwarya Singh Rathore · Detection, Diagnosis and Management of Air-Borne Diseases in Agricultural Crops · 2025
A.G. Meena, C. Gopalakrishnan, A. Kamalakannan · Physiological and Molecular Plant Pathology · 2025
Antonin Douillet, Benoit Laurent, Jessie Beslay · Journal of Applied Microbiology · 2022
Marta Maria Barone, Marco Moizio, Ravish Choudhary · Journal of Fungi · 2026
Joaquín Balduque-Gil, Ana Garcés-Claver, Inés Pérez-Lamuela · Agronomy · 2024
Govindan Muthukumar, Ayyanar Kamalakannan, Irudhayasamy Johnson · Physiological and Molecular Plant Pathology · 2025
Showing 8 of 24 known citations — external sources report more than can currently be individually listed.
Related research
- Prevalence of Fusarium Species Associated with Peach Decline in Tunisian Nurseries — shares topic coverage
- Biochemical and Molecular Characterization of Bacterial Wilt Disease of Banana and Evaluation of their Antibiotic Sensitivity — shares topic coverage
- Detection of Sutterella in the Stool of Egyptian Children with Autism Spectrum Disorders — shares topic coverage
- Incidence of Heat-Labile Toxin (LT) Producing Enterotoxigenic Escherichia coli Mediated Diarrheal Disease in Egyptian Children under 5 Years Presenting to Tanta University Hospital — shares topic coverage
- Molecular Characterization and Detection of Infection in Vector Snails of Urinary Schistosomiasis around Erinle and Eko Ende Dams in South West Nigeria — shares topic coverage
Article metrics
Real usage data collected on this platform.
68
Page views
0
PDF downloads
34
Outbound clicks
24
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.