Loop-Mediated Isothermal Amplification (LAMP): Comparative Advances over Conventional PCR and Other Molecular Techniques
Yahaya Hassan, Leslie Thian Lung Than
Annual Research & Review in Biology · pp. 33–44 · Published 8 Aug 2020
10.9734/arrb/2020/v35i830257Abstract
Gene amplification technology is essential in the fields of diagnostic medicine. The polymerase chain reaction (PCR) is central in the molecular studies and provides ways for diagnostic advancement in the areas. However, the requirement for thermal cycler in a dedicated facility for amplification of target genes in the PCR technique has been a bottleneck to many researchers. The limitations associated with PCR include cost implication, strict expertise necessity and relatively higher turn-around time. The emergence of loop-mediated isothermal amplification (LAMP) in the last two decades assists in bridging the undesirable gaps. This review aims to highlight the natural advantages of the LAMP technique over the existing conventional PCR and other isothermal molecular techniques. Available published articles on LAMP techniques reviewed, listed many outstanding advances of the method in comparison to traditional PCR technique. The mentioned advantages include simplicity, affordability, naked-eye result detection and many more. That made LAMP becomes a rapidly accepted method in the field of molecular diagnosis. Other essential features of LAMP in comparison with other emerging nucleic acid amplification techniques were adequately explained and presented in tabular form for research and quick reference purposes. Though LAMP has some few limitations, its advantages outweigh its flaws by filling the gap in the field of molecular biology diagnostics.
Cited by 11
Xiaoxiao Ma, Xun Ma, Yingying Ma · RSC Advances · 2025
Mateen Nawaz, Tingting Gao, Kejing Huang · Aquaculture Research · 2022
Ye-Ji Moon, So-Young Lee, Unji Kim · Food Science and Biotechnology · 2023
Sujesh Sudarsan, Anusha Prabhu, Dinesh Prasad · The Analyst · 2023
Isabelle Robène, Véronique Maillot-Lebon, Yann Pecrix · PhytoFrontiers™ · 2023
So Yeon Park, Dong-Sik Chae, Jae Sun Lee · Biosensors · 2023
O. Yugovich, M. Bunce, SA. Harbison · Forensic Science International: Genetics · 2025
Jethro Vernon, Pep Canyelles-Pericas, Hamdi Torun · IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control · 2022
Dongmin Kim, Terry J. DeBriere, Bradley H. Eastmond · PLOS ONE · 2024
Cantika Aprillia, Aliya Azkia Zahra, Jekmal Malau · Journal of Pharmaceutical and Sciences · 2026
Related research
- Contribution of InvA Gene PCR to Recovery of Salmonella spp. Strains — shares topic coverage
- Molecular Detection of New Delhi Metallo Beta Lactamase 1 (NDM-1) Producing Bacterial Isolates in Kano- Northwestern Nigeria — shares topic coverage
- Genetically Modified Crops and Food Security in Nigeria; Facts and Myths — shares topic coverage
- Production of Biodiesel from Marine and Freshwater Microalgae: A Review — shares topic coverage
- A Generous Review: Novel Approaches for Colon Targeted Drug Delivery System — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
11
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.