Molecular Characterization of Penicillium Isolates Using Rapd Technique

Abstract

Molecular analyses were performed on 14 isolates of Penicillium species. The Penicillium isolates examined were collected from different localities and habitats of Jeddah, Saudi Arabia and maintained on Czapek Dox's and Waksman's media. Random Amplified Polymorphic DNA (RAPD-PCR) technique was used in this study to distinguish these isolates. A total of 42 DNA bands were generated by these primers in the Penicillium isolates grown on Czapek Dox's medium while, Penicillium isolates grown on Waksman's medium, 43 DNA bands were generated by the primers. Of the polymorphic bands that were identified in the Penicillium isolates grown on Czapek Dox's medium, two were unique, while no such bands could be detected in the Penicillium isolates grown on Waksman's medium. Penicillium isolates grown on both Czapek Dox's and Waksman's media, 40 monomorphic bands were detected. These monomorphic bands were used to distinguish among the studied Penicillium isolates under study. Data were analysed by a clustering method and similarity coefficients using NTSYSpc version 2.02i. The relationships between the species and isolates are discussed.