Skip to content
Research Article Open access CC BY 4.0

Protocol Optimization of DNA Extraction from Banana Fruits

Denis Omara, Banson John Barugahare

Journal of Advances in Biology & Biotechnology · pp. 1–5 · Published 23 Jun 2022

10.9734/jabb/2022/v25i430274

Abstract

Background: DNA extraction is the process by which the DNA is extracted and purified from the living or dead cells or tissues. There are many different kits commercially available for DNA extraction, with varying protocols. This study aimed at determining the optimal conditions for the extraction of high yield DNA from banana fruits. Methodology: DNA was extracted from banana fruits by precipitation method. The DNA yield was determined using the Nanodrop DNA quantification technique. Results: The optimal sodium chloride salt concentration for DNA extraction from banana fruits was 4mM and the amount of DNA extracted increased as the temperature decreased. The highest yield was obtained between 4 0C to 0 0C. Conclusion: We report for the first time a protocol with an optimal salt concentration (NaCl, 4mM) for quality DNA yield from banana fruits.  A critical consideration is required to establish an optimal range of moderate temperatures and DNA concentrations for better quality and desired yields. There may not be a universal protocol for DNA extraction from all plant materials.

DNA extraction banana fruits sodium ion salt concentration temperature

References (18)

  1. 1 DNA, RNA, and Protein Extraction: The Past and The Present [DOI]
  2. 2 Nucleic Acid Helix Stability: Effects of Salt Concentration, Cation Valence and Size, and Chain Length [DOI]
  3. 3 Controlling the size of nanoscale toroidal DNA condensates with static curvature and ionic strength [DOI]
  4. 4 Rapid and Efficient Isolation of High Quality Nucleic Acids from Plant Tissues Rich in Polyphenols and Polysaccharides [DOI]
  5. 5 Comparison of DNA extraction kits for PCR-DGGE analysis of human intestinal microbial communities from fecal specimens [DOI]
  6. 6 Factors and methods to modulate DNA hybridization kinetics [DOI]
  7. 7 An efficient protocol for total DNA extraction from the members of order Zingiberales- suitable for diverse PCR based downstream applications [DOI]
  8. 8 The temperature dependence of the helical twist of DNA [DOI]
  9. 9 Short Communication An efficient method for simultaneous extraction of high-quality RNA and DNA from various plant tissues [DOI]
  10. 10 Effects of Concentration and Temperature on DNA Hybridization by Two Closely Related Sequences via Large-Scale Coarse-Grained Simulations [DOI]
  11. 11 A Simple and Efficient DNA Isolation Method for Salvia officinalis [DOI]
  12. 12 Optimization of DNA isolation and PCR protocol for RAPD analysis of banana / plantain (Musa spp.) [DOI]
  13. 13 Establishment of a rapid, inexpensive protocol for extraction of high quality RNA from small amounts of strawberry plant tissues and other recalcitrant fruit crops [DOI]
  14. 14 A Comparative Study of Some Procedures for Isolation of Fruit DNA of Sufficient Quality for PCR-Based Assays [DOI]
  15. 15 DNA extraction techniques for use in education [DOI]
  16. 16 Development of an effective and efficient DNA isolation method for Cinnamomum species [DOI]
  17. 17 An introduction to molecular biotechnology
  18. 18 Determining the optimal method for DNA isolation from fruit jams [DOI]

Cited by 2

Development and Characterization of a Cationic Polyurethane Carrier for Enhanced Topical DNA Delivery

Florin Borcan, Adel Len, Titus Vlase · Drug Design, Development and Therapy · 2025

Showing 1 of 2 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

13

Page views

0

PDF downloads

1

Outbound clicks

2

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.