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Research Article Open access CC BY 4.0

Protective Role of Walnut Seeds Extract and Vitamin E against Testicular Toxicity Induced by Cyclophosphamide in Male Rats

Aml F. Elgazar

Journal of Advances in Medicine and Medical Research · pp. 1–10 · Published 11 Nov 2016

10.9734/BJMMR/2016/29765

Abstract

Aim: The protective role and antioxidant effect of Walnut seeds extract (WSE) and/or vitamin E (Vit. E), and their combination against testicular damage and oxidative stress induced by anticancer drug cyclophosphamide (CP), were studied on male rats. Study Design: Forty five rats were randomized into five equal groups, of nine rats each. Group I was assigned as a negative control and orally received saline solution. The other four groups were given CP orally (dissolved in saline solution) at a daily dose of 5 mg/kg of body weight (b. wt) for six weeks. Group II was used as a positive control and groups III, IV and V were received WSE (400 mg/kg of b. wt), Vitamin E (200 mg/kg b. wt) and their combination in daily doses for six weeks, respectively, four hours after CP administration. Methods: Initial and final weight of rat, food intake were recorded and weight gain and feed efficiency ratio of rats were calculated and recorded. At the end of experimental, rats were anaesthetized by diethyl ether; testes and accessory sexual organs were dissected out and weighed. Semen was separated and analyzed, serum levels of testosterone, FSH and LH were determined. The right testes were used for estimating testicular antioxidant capacity. The left testes were preserved in 10% formalin solution untills processing for histopathological examination. Results: Oral administration of WSE, vitamin E and their combination to intoxicated rats caused increases in the weights of testes, seminal vesicles, sperm motility, count and viability. Serum testosterone and levels of FSH and LH were increased. There were significant increases in the activities of testicular antioxidant enzymes, and alleviation of testicular degeneration and necrosis induced by CP. These effects were amplified by combination of WSE with vitamin E. Conclusion: Combination of Walnut seeds extract with vitamin E exhibited antioxidant activity and protective effect against reproductive toxicity induced by CP in male rats. Therefore, intake of Walnut seeds in food with vitamin E as food supplement may be beneficial for male patients suffering from infertility problems.

Walnut seeds vitamin E cyclophosphamide testes testosterone sperms antioxidant histopathology.

References (26)

  1. 1 Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase.
  2. 2 GONADAL DAMAGE FROM CHEMOTHERAPY AND RADIOTHERAPY [DOI]
  3. 3 The association of age and semen quality in healthy men [DOI]
  4. 4 Antioxidative polyphenols from walnuts (Juglans regia L.) [DOI]
  5. 5 Applied animal reproduction
  6. 6 Protection of cyclophosphamide-induced toxicity in reproductive tract histology, sperm characteristics, and DNA damage by an herbal source; evidence for role of free-radical toxic stress [DOI]
  7. 7 Antioxidative effects of melatonin on kinetics, microscopic and oxidative parameters of cryopreserved bull spermatozoa [DOI]
  8. 8 Potential chemoprotective effect of melatonin in cyclophosphamide- and cisplatin-induced testicular damage in rats [DOI]
  9. 9 Cytoprotective effects of DL-alpha-lipoic acid or squalene on cyclophosphamide-induced oxidative injury: An experimental study on rat myocardium, testicles and urinary bladder [DOI]
  10. 10 Rutin ameliorates cyclophosphamide-induced reproductive toxicity in male rats [DOI]
  11. 11 Effect of $alpha;-tocopherol and simvastatin on male fertility in hypercholesterolemic rats [DOI]
  12. 12 Medical treatment to improve sperm quality [DOI]
  13. 13 Safety and efficacy of Zingiber officinale roots on fertility of male diabetic rats [DOI]
  14. 14 Walnuts Improve Semen Quality in Men Consuming a Western-Style Diet: Randomized Control Dietary Intervention Trial1 [DOI]
  15. 15 Effects of α‑tocopherol and ascorbic acid in the severity and management of traumatic brain injury in albino rats [DOI]
  16. 16 Study on antioxidant activity of common dry fruits [DOI]
  17. 17 Dose‐response effect of ellagic acid on circulatory antioxidants and lipids during alcohol‐induced toxicity in experimental rats [DOI]
  18. 18 Beneficial effects of american ginseng on epididymal sperm analyses in cyclophosphamide treated rats.
  19. 19 Crataegus monogyna aqueous extract ameliorates cyclophosphamide-induced toxicity in rat testis: stereological evidences.
  20. 20 Protective effect of aminoguanidine against cyclophosphamide-induced oxidative stress and renal damage in rats [DOI]
  21. 21 Protective effect of diallyl disulfide on cyclophosphamide-induced testicular toxicity in rats [DOI]
  22. 22 Effect of administration of vitamins C and E on fertilization capacity of rats exposed to noise stress [DOI]
  23. 23 Chemoprotective potential of Coccinia indica against cyclophosphamide-induced toxicity [DOI]
  24. 24 Penile alterations with severe sperm abnormalities in antiphospholipid syndrome associated with systemic lupus erythematosus [DOI]
  25. 25 Antioxidant activity comparison of walnuts and fatty fish.
  26. 26 Protective effect of Juglans nigra on sodium arsenite-induced toxicity in rats [DOI]

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