Production and Application of No-purified Rhamnolipids in the Soil-washing of TPHs Contaminated Soils
Luis G. Torres, Roberto González, Jorge Gracida
Asian Soil Research Journal · pp. 1–12 · Published 19 Apr 2018
10.9734/asrj/2018/v1i1618Abstract
Aims: This work aimed to demonstrate the feasibility of producing (mono- and di-) rhamnolipids employing a strain of Pseudomonas aeruginosa strain ATCC 9027 employing olive oil as a substrate and some mineral salts. This rhamnolipid is a biosurfactants with multiple applications The CMC of this product under different conditions (filtered, unfiltered, in the presence and absence of Fe and Mg, at different pH values) was assessed. At the end, the UP was assessed in the washing of a TPH contaminated soil. Place and Duration of Study: Bioprocess department. Unidad Profesional Interdisciplinaria de Biotecnologia-IPN facilities, during 2016. Methodology: Rhamnolipids were produced with P. aeruginosa in olive oil, then by drying the culture broth was generated an unpurified product (UP) that contained 0.19% rhamnolipids. Critical micelle concentration CMC of UP products were evaluated in the presence of Ca2+ or Fe3+ from 0.5 to 2 mM, and pH values from 4 to 10. Finally, this surfactant was assessed in the washing of hydrocarbon-contaminated soils, and compared with other synthetic surfactants. Results: It was found that CMCs were similar to those reported in the literature for pure rhamnolipids. The UP products have shown dynamic behavior in the soil washing at concentrations below 176 mg/L because removed 80% of 6,500 mg TPH/Kg from a gravel-sandy soil; the rhamnolipids could be removed TPH through mobilization mechanism. Conclusion: It was possible to produce rhamnolipid using olive oil as carbon source and strain of P. aeruginosa ATCC 9027 to levels of 100 mg/L. It was feasible to produce a powder containing 1.19% of rhamnolipids. The UP had better properties as a surfactant than the purified product. The pH affects the CMC of the rhamnolipids in a way that promotes their behavior as ionic surfactant or nonionic surfactant. The ionic strength with Ca 2+ and Fe3+ has an effect on the CMC of rhamnolipids so that the decreases in the range of 35 to 41 mg/L in the presence of 0.5 to 2 mM of metals. The UP rhamnolipids were employed for washing soil contaminated with 6,500 mg/kg increased TPH removal at low concentrations and to be as effective as chemical surfactants. TPH removal observed was about 80% for rhamnolipid with a CMC x 0.074 concentration.
References (23)
- 1 Rhamnolipids: diversity of structures, microbial origins and roles [DOI]
- 2 An overview and analysis of site remediation technologies [DOI]
- 3 Towards commercial production of microbial surfactants [DOI]
- 4 Biosurfactant-enhanced removal of total petroleum hydrocarbons from contaminated soil [DOI]
- 5 Evaluation of biosurfactants for crude oil contaminated soil washing [DOI]
- 6 Chemical structure, surface properties and biological activities of the biosurfactant produced by Pseudomonas aeruginosa LBI from soapstock [DOI]
- 7 Microbial production of rhamnolipids: opportunities, challenges and strategies [DOI]
- 8 Engineering bacteria for production of rhamnolipid as an agent for enhanced oil recovery [DOI]
- 9 A comparison of the efficiency of different surfactants for removal of crude oil from contaminated soils [DOI]
- 10 Why do microorganisms produce rhamnolipids? [DOI]
- 11 Stability Constants for the Complexation of Various Metals with a Rhamnolipid Biosurfactant [DOI]
- 12 Washing as a remediation technology applicable in soils heavily polluted by mining–metallurgical activities [DOI]
- 13 Toxicity comparison of biosurfactants and synthetic surfactants used in oil spill remediation to two estuarine species [DOI]
- 14 Effect of electrolytes on the surface behavior of rhamnolipids R1 and R2 [DOI]
- 15 Molecular and structural characterization of the biosurfactant produced by Pseudomonas aeruginosa DAUPE 614 [DOI]
- 16 Thermodynamics of the Interaction of a Dirhamnolipid Biosurfactant Secreted by Pseudomonas aeruginosa with Phospholipid Membranes [DOI]
- 17 Reuse of waste frying oil for production of rhamnolipids using Pseudomonas aeruginosa zju.u1M [DOI]
- 18 High production of rhamnolipids by Pseudomonas aeruginosagrowing on ethanol [DOI]
- 19 Washing of Soil Contaminated with PAHs and Heavy Petroleum Fractions Using Two Anionic and One Ionic Surfactant: Effect of Salt Addition [DOI]
- 20 Characterization and treatability of a contaminated soil from an oil exploration zone [DOI]
- 21 Surfactant-Enhanced Washing of Soils Contaminated with Wasted-Automotive Oils and the Quality of the Produced Wastewater [DOI]
- 22 Purification and structural characterization of glycolipid biosurfactants fromPseudomonas aeruginosa YPJ-80 [DOI]
- 23 A facile reactor process for producing 7,10-dihydroxy-8(E)-octadecenoic acid from oleic acid conversion by Pseudomonas aeruginosa ** [DOI]
Cited by 10
Gabriele Beretta, Marta Puddu, Sabrina Saponaro · Reviews in Environmental Science and Bio/Technology · 2026
Sahar Kalvandi, Hamidreza Garousin, Ahmad Ail Pourbabaee · Scientific Reports · 2022
Varsha Singh, Zairah Waris, Sriparna Saha · Microbes and Microbial Biotechnology for Green Remediation · 2022
Showing 3 of 10 known citations — external sources report more than can currently be individually listed.
Related research
- Pseudomonas monteilii and Citrobacter murliniae, Biosurfactant-Producing Bacteria Isolated from Nigerian Soil — shares topic coverage
- Potentials for Biosurfactant Enhanced Bioremediation of Hydrocarbon Contaminated Soil and Water–A Review — shares topic coverage
- Effect of Biosurfactants Extracted from a Locally Fermented Milk (Pendidam) on Its Shelf Life — shares topic coverage
- Biofilm and Biosurfactant Mediated Aromatic Hydrocarbons Degradation by Marine Bacteria Isolated from Contaminated Marine Environments of Niger Delta — shares topic coverage
- Biosurfactant Production by Fusarium oxysporum LM 5634 Using Peels from the fruit of Bactris gasipaes (Kunth) as Substrate — shares topic coverage
Article metrics
Real usage data collected on this platform.
40
Page views
0
PDF downloads
4
Outbound clicks
10
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.