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Research Article Open access CC BY 4.0

Prevalence of Methicillin Resistant Staphylococcus aureus among Isolates from Wounds in Surgical Wards at Kabale Regional Referral Hospital, South Western Uganda

Baguma Andrew, Kiiza Aidah, Joel Bazira Bazira

Microbiology Research Journal International · pp. 1–5 · Published 17 Nov 2016

10.9734/BMRJ/2016/27807

Abstract

Background: Methicillin resistant S. aureus (MRSA) is a multidrug resistant organism that threatens the continued effectiveness of antibiotics worldwide and causes a threat almost exclusively in hospitals and long-term care settings. This study sought to determine the prevalence and antimicrobial susceptibility pattern of MRSA at Kabale Regional Referral Hospital (KRRH) in South Western Uganda. Methods: This was a cross sectional study conducted between June and November 2014 involving 350 pus swabs from infected surgical sites. The samples were cultured on Mannitol salt agar at 37°C for 17- 24 hours and the colonies were tested and confirmed as S. aureus using API staph testing kit. Screening for MRSA was performed using the Cefoxitin (30 µg) disc on Muller Hinton agar medium. MRSA susceptibility was performed by the Kirby Bauer disc diffusion on MHA for the following antibiotics: Ceftriaxone, Ciprofloxacin, Erythromycin, Imipenem, Trimethoprim – sulfamethoxazole, Levofloxacin and Gentamycin. The zones of inhibition were measured using a ruler and compared with the CLSI guidelines 2012. Results: From the 350 pus swabs, 331 isolates of S. aureus were obtained, of which 179 (54%) isolates were Methicillin resistant. Majority (54%) of the MRSA isolates were highly sensitive to Gentamycin and Levofloxacin but highly resistant to Ceftriaxone (100%), Imipenem (100%), Trimethoprim – sulfamethoxazole (85%), and Erythromycin (67%). Conclusion: The prevalence of MRSA in KRRH is high with high resistance to Ceftriaxone, Imipenem, Trimethoprim – sulfamethoxazole and Erythromycin. However, good sensitivity to gentamycin and Levofloxacin is still observed.

Methicillin resistant Staphylococcus aureus (MRSA) Kabale Regional Referral Hospital (KRRH) surgical ward.

References (5)

  1. 1 Community-Associated Methicillin-Resistant Staphylococcus aureus : Epidemiology and Clinical Consequences of an Emerging Epidemic [DOI]
  2. 2 Methicillin-Resistant Staphylococcus aureus (MRSA) Nares Colonization at Hospital Admission and Its Effect on Subsequent MRSA Infection [DOI]
  3. 3 Relative prevalence of methicilline resistant Staphylococcus aureus and its susceptibility pattern in Mulago Hospital, Kampala, Uganda [DOI]
  4. 4 Short communication: Identification of coagulase-negative staphylococcus species from goat milk with the API Staph identification test and with transfer RNA-intergenic spacer PCR combined with capillary electrophoresis [DOI]
  5. 5 Prevalence and Antibiotic Susceptibility Patterns of Clinical Isolates of Methicillin-Resistant Staphylococcus aureus in a Tertiary Care Hospital in Western Uganda [DOI]

Cited by 2

Prevalence of Nasal Carriage of Methicillin Resistant Staphylococcus Aureus Among Patients, Health Care Workers and Patients’ Care Takers at Kabale Regional Referral Hospital, South Western Uganda

· Archives of Clinical and Medical Microbiology · 2023

Prevalence of Nasal Carriage of Methicillin Resistant Staphylococcus aureus among Patients, Health Care Workers and Patients’ Care Takers at Kabale Regional Referral Hospital, South Western Uganda

Jude Collins Busingye, Joel Bazira, Benson Musinguzi · Research Square · 2023

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