Prevalence and Antibiotic-resistant Patterns of Listeria spp. Isolated from Faeces of Dairy Cattle in Selected Areas of Bangladesh

Abstract

The study aimed to investigate the epidemiology and antibiogram of Listeria species isolated from dairy cattle of Bangladesh. Faecal samples (300) were collected aseptically from randomly selected dairy farms of Dhaka, Barishal, and Mymensingh divisions (100 from each) of Bangladesh from July 2023 to June, 2024. The samples were primarily enriched in Listeria enrichment broth and isolated on Polymyxin Acriflavin Lithium-chloride Ceftazidime Esculin Mannitol (PALCAM) agar. The identification of Listeria spp. was performed based on morphology, cultural and biochemical characteristics. The identified Listeria spp. were then tested for antibiotic sensitivity. The overall prevalence of Listeria spp. from faecal sample of dairy cattle was 9.33% whereas prevalence of 8.00%, 9.00%, and 11.00% were observed for Dhaka, Barishal, and Mymensingh, respectively. Ampicillin, amoxicillin, and amoxycillin/clavulanic acid (each of 96.43%) showed high resistance to the isolated Listeria spp. followed by erythromycin (89.29%) and tetracycline (85.71%) while, gentamicin (96.43%), meropenem (96.43%), ciprofloxacin (71.43%), and ceftriaxone (60.71%) were sensitive to the isolated Listeria spp. Care therefore should be taken to prevent the development of multidrug resistant Listeria spp. in dairy cattle due to its public health significance.