Preparation of Mouthwash Using Blue Tea and Its Antioxidant Activity

Abstract

Introduction: Clitoria ternatea also known as the "blue pea," is a perennial twinning herbaceous plant in the Fabaceae family. The medicinal properties of this plant have been scientifically validated, especially at an international level, and it has been recorded to have a variety of biological activities, including antioxidant, antidiabetic, and hepatoprotective properties. Aim: The aim of this study was to evaluate the antioxidant property of blue tea extract based mouthwash. Materials and Methods: The study was performed as an in vitro study under a laboratory setting. Synthesis of the mouthwash was performed using dried leaves of the butter pea plant subsequently the mouthwash was tested for its antioxidant activity and was assessed via the DPPH assay. The obtained values were compared with that of a known standard. The obtained data was sorted in Microsoft Excel and statistically analyzed using Statistical Package for Social Sciences (SPSS Software, Version 23.0). Unpaired t test was done for the comparison of blue tea extract with the standard and one way ANOVA followed by Tukey’s post hoc test was done to compare various concentrations with standard. Results: The blue tea extract mouthwash was tested with 10µL initially and then was increased to 20µL, 30µL, 40µL and 50µL. Based on DPPH assay, as the concentration of the extract solution was increased by 10µL each time tested, their antioxidant activity also increased over a scale. It was seen that the blue tea extract mouthwash had significantly less antioxidant capacity when compared to the standard (p<0.05). Conclusion: The blue tea extract mouthwash is found to be a potent antioxidant agent however the antioxidant property was less effective when compared to the standard.